摘要
采用高效液相色谱法检测食品中多种香料共存时的罂粟壳。样品经碱化后采用氯仿 乙醇 (体积比为 9∶1)提取 ,在苯基柱上以乙腈 甲醇 水为流动相进行分离 ,外标法定量。罂粟碱 ,吗啡 ,可待因在 1 2mg/L～ 10 0mg/L范围内线性关系良好 ,平均回收率为 79.3%～ 86 .1%。
An HPLC method for the determination of Pericarpium papaveris in foods accompanied with some fragrant substances is described. The Pericarpium papaveris in food was extracted in alkaline condition with CHCl\-3 C\-2H\-5OH (9∶1, V/V )and analyzed by reversed phase HPLC by using a YWG C\-6H\-5 column and acetonitrile methanol water as mobile phase.The calibration curves of papaverine, morphine and codeine were linear in the range of 1.2 mg/L 100 mg/L and the mean recoveries were from 79.3% to 86.1% . The method has a good specificity and accuracy.
出处
《色谱》
CAS
CSCD
北大核心
2000年第6期554-555,共2页
Chinese Journal of Chromatography
关键词
高效液相色谱法
食品
罂粟壳
检测
high performance liquid chromatography
food
Pericarpium papaveris
