摘要
应用反相高效液相色谱外标定量法同时测定了舒普深中的舒巴坦钠和头孢哌酮钠。采用 pH 4 0的磷酸水溶液 乙腈 (体积比为 80∶2 0 )组成的流动相 ,在ODS柱上 ,于 12min内同时测定舒巴坦钠和头孢哌酮钠。方法简单 ,重现性好 ,在线性范围内舒巴坦钠的相关系数为 0 9997(n =5 ) ,头孢哌酮钠的相关系数为 0 9991(n=5 )。
A reversed phase, isocratic high performance liquid chromatographic method with acid mobile phase can separate sulbactam and cafoperazone within 12 minutes Column packed with Hypersil ODS2(250 mm×4 6 mm i d , 5 μm) was manufactured by Dalian Elite Company Mobile phase is composed of water (adjusted to pH 4 0 with 1% phosphoric acid) and acetonitrile (80∶20, V/V ) The detection was performed at 210 nm and the injection volume was 2 μL Cefoperazone and sulbactam have good linearity in the ranges of 100 mg/L to 800 \{mg/L\} and 100 mg/L to \{1 000\} mg/L with the correlation coefficients of \{0 999 1\} and \{0 999 7\} respectively. This method is easily to be operated and can be applied for manufacturing and medicinal study.
出处
《色谱》
CAS
CSCD
北大核心
2000年第6期525-526,共2页
Chinese Journal of Chromatography
关键词
舒普深
舒巴坦钠
头孢哌酮钠
测定
HPLC
high performance liquid chromatography
Sulperazon
sulbactam sodium
cefoperazone sodium(
