摘要
建立了测定大豆和葛根７种异黄酮成分的高效液相色谱法。采用日本岛津Ｓｈｉｍ－Ｐａｒｋ ＣＬＣ ＯＤＳ柱（１５０ ｍｍ ｘ ６．０ ｍｍ ＩＤ；５μｍ），Ｖ 甲醇：Ｖ醋酸： Ｖ水＝ ３０：３．５：６６． ５体系为流动相，０～８． ５ｍｉｎ对流动相流速为 １． ０ ｍＬ／ｍｉｎ，８．５～ ４７ｍｉｎ时流速为１．５ｍＬ／ｍｉｎ，柱温５０℃ ，检测波长 ２５４ｎｍ，外标法定量。
Seven isoflavones in soybeans and Radix Puerariae were determined by HPLC with a Shim- Pack CLC ODS column(150 mm×6. 0 mm ID; 5 μm)and UV detector at 254 nm. The MeOH - HAc- H2O system(30: 3. 5: 66. 5 by volume) was used as a mobile phase. The flow rate was 1.0 mL/min in the period from 0 to 8.5 min and 1.5 mL/min from 8.5 to 47 min. Column temperature was 50℃. An external standard method was used for the quantitative analysis.
出处
《分析测试学报》
CAS
CSCD
2000年第6期28-31,共4页
Journal of Instrumental Analysis
