摘要
制备一种吖啶-4-酰胺类探针9-[(N-2-二甲氨乙基)吖啶-4-酰胺]-α-丙氨酸(NACA),研究NACA与牛血清蛋白(BSA)的结合作用.测定了Stern-Volmer猝灭常数KSV、热力学参数(ΔH、ΔG及ΔS)、结合位点数n、表观结合常数KA和NACA-BSA作用距离r,确定了NACA对BSA的荧光静态猝灭机制和主要作用力.
An acridine - 4 - carboxamide skeleton - based probe N - （9 - （ N - （ 2 - dimethylamino） ethyl） acridine - 4 - carboxamide） - α - alanine （NACA） was synthesized. The interaction between NACA and bovine serum albumin （BSA） was investigated. The Stern - Volmer fluorescence quenching constant （Ksv） was investigated, as well as thermodynamics constants of △H, △G and △S. Other param- eters including the number of combining sites （ n）, apparent combining constants （ KA ） and interaction distance （r） of NACA -BSA were estimated. The static quenching was found to cause the fluorescence quenching of BSA and the interaction mechanism between NACA and BSA was also discussed.
出处
《福州大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第5期940-946,共7页
Journal of Fuzhou University(Natural Science Edition)
基金
国家自然科学基金资助项目(21177022)
"十二五"国家科技支撑资助项目(2012BAK08B06-1)
