摘要
本文报道了用胆固醇氧化酶和胆固醇酯酶制备的酶膜,与氧电极偶合的电极法,结合流动注射分析法,直接测定人体血清总胆固醇,方法准确、快速,线性范围为6.67×10^(-4)mg/ml～1.20×10^(-2)mg/ml,工作曲线的线性相关系数为0.9993,测定的相对标准偏差为1.63%。
Direct determination of total cholesterol in human serum is accomplished by flow injectionanalysis-enzyme electrode. The enzyme membrane used in this method is prepared from choles-terinase and cholesterol oxidase. Cholesterol in concentration range of 6.67×10^(-4)mg/ml--1.20×10^(-2)mg/ml can be determined by this method with a relative standard deviation of 1.63% andthe calibration curve has a liner correlation coefficient of 0.9993.
关键词
FIA
酶电极法
胆固醇
人体
血清
Enzyme electrode
flow injection analysis
total cholesterol
