摘要
本文报道由作者建立的薄层色谱法,样品在硅胶薄层板上展开后,以苯芴酮显色,再在碘钨灯下照射可使锗-132衍生成荧光化合物,从而可用原位荧光扫描(外标法)定量,已用于倍半氧双羧乙基锗(锗-132)原料和制剂的含量测定及稳定性研究。经3批原料、9批注射液、3批口服液样品的222个测试数据统计,C.V.值为2.29%,同板的C.V.值为1.5%～2.7%(n=7～10);加样回收率101.5%,最低检出限为0.12μg。
Organic Germanium, also known as Ge-132, has been recent years introduced in China as anutritional supplement. For the need of quality control and stability, study a fluorodensitometric thinlayer chromatographic method for quantitative analysis of organic germanium has been establishedin our laboratory based on postchromatographic fluorescence derivatization with dipping the chro-matogram into phenylfluorone reagent, followed by irradiation under quartz halogen lamp. Theadvantages of this unprecedented TLC method over the conventional titration and atom absorptionare high sensitivity, good reliability and speciality.
关键词
锗-132制剂
含量
测定
薄层色谱法
Organic germanium
Ge-132
fluorodensitometric TLC
quantitative analysis
