摘要
目的:研究耳叶牛皮消苷A总代谢物(MAA)对肿瘤细胞的抑制作用及诱导肿瘤细胞凋亡机制。方法:采用MTT法,观察MAA对肿瘤细胞的抑制率以及IC_(50)评价其体外抗肿瘤作用;通过流式细胞术(FCM)考察MAA的诱导肿瘤细胞凋亡机制。结果:MAA对多种肿瘤细胞具有显著抑制作用;且能诱导PC-3细胞凋亡,使肿瘤细胞周期阻滞在G2/M期。结论:MAA具有显著的抗肿瘤作用,且具有诱导肿瘤细胞凋亡的能力。
Objective ： To observe the growth inhibitory effect of tumor cells and apoptosis mechanism by auriculoside A metabolic product （MAA）. Methods ：The anti - proliferative activities of MAA against a quantity of tumor cells were eval- uated by MTT colorimetric assay in vitro. The result showed by inhibitory rate and IC50. The apoptosis mechanism of MAA on PC - 3 was observed by FCM. Results ： MAA had significant anti - proliferative activities against tumor ceils in vitro. MAA can induce apoptosis on PC -3 cell line, and make the cells arrest in G2/M phase. Conclusion：MAA has sig- nificant growth inhibitory effect and the ability of induce apoptosis on tumor cells.
出处
《中华中医药学刊》
CAS
2013年第11期2507-2509,I0008,共4页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省医药卫生科学研究基金项目(2011KYA007)
关键词
耳叶牛皮消苷A
代谢物
抗肿瘤
auriculoside A
metabolic product
anti - tumor
