摘要
目的 探讨胃癌组织和癌旁组织中微小RNA（miR）-10b的表达差异及其临床意义.方法 采用SYBR Green Ⅰ荧光定量聚合酶链反应（FQ-PCR）法检测78例胃癌患者手术切除癌组织及配对癌旁组织标本中miR-10b的表达水平.结果 miR-10b在胃癌组织中的相对表达水平（6.758±2.301）显著高于其在配对癌旁组织中的表达（8.516±1.870）,差异有统计学意义（P＜0.01）.胃癌组织中miR-10b的表达水平与临床TNM分期（P＜0.01）、肿瘤大小（P＜0.01）、淋巴结转移（P＜0.01）以及肿瘤浸润深度（P＜0.01）密切相关,而与年龄、性别、分化程度无明显相关（P＞0.05）.结论 胃癌组织中miR-10b的高表达可能与胃癌的发生、发展、侵袭、转移相关.
Objective To investigate the expression of microRNA （miR）-10b in gastric carcinoma and the clinical significance.Methods Seventy-eight pairs of human gastric carcinoma tissue samples were obtained from patients who underwent surgical resection.The level of miR-10b in 78 gastric carcinoma and the adjacent normal tissues was quantified by SYBR Green Ⅰ real-time reverse transcription polymerase chain reaction （FQ-PCR）,and the relationship between miR-10b and clinicopathological features in gastric carcinoma was explored.Results The expression of miR-10b in gastric carcinoma tissues （6.758 ±2.301） was significantly increased as compared with that in the adjacent normal tissues （8.516 ± 1.870,P ＜ 0.01）.The evaluated level of miR-10b was associated with TNM classification （P ＜ 0.01）,tumor size （P ＜ 0.01）,lymph node metastases （P ＜ 0.01） and local invasion in gastric carcinoma （P ＜ 0.01）,but not with sex,age,and differentiation （all P ＞ 0.05）.Conclusion These findings suggested miR-10b may play important roles in the development,progression,invasion and metastasis of gastric carcinoma.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第10期2203-2205,共3页
Chinese Journal of Experimental Surgery
基金
浙江省自然科学基金资助项目（Y2101444）
