摘要
将60只健康试验大鼠随机分为3组:正常对照组(A组)、ET处理组(B组)、CA+ET处理组(C组),运用PCR-SSCP技术对肝脏mtDNA tRNACys-Tyr、COI基因片段一等基因进行了分析,同时运用分光光度计法对血清丙二醛的含量进行测定,观察ET致mtDNA的突变效应以及CA对该效应的保护作用。,结果发现,B组mtDNA tRNACys-Tyr基因片段在第5238与5242碱基处发生了插入1个碱基T的插入突变,COI一基因片段发生T5322G和T5331G处置换点突变,其他各组无基因突变发生。B组血清MDA含量均明显高于其他二组(P<0.05)、C组明显低于B组但高于A组(P<0.05)。结果表明,ET可导致mtDNA基因发生突变,而CA对ET致基因突变具有保护效应。
To investigate the mutational effect of endotoxin （ET）-induced mtDNA and the protec- tive effect of cation A （CA）,sixty experimental rats were divided randomly into 3 groups：normal control group （group A）,ET treated group （group B）,and CA＋ET treated group （group C）. The liver mtDNA was extracted for detecting the mutations of tRNAcys-Tyr ,COI fragment one gene by polymerase chain reaction-single stranded conformation polymorphism （PCR-SSCP） tech- nique. The results revealed that a mutation was tRNAcys-Tyr of group B,which was inserted T be- tween 5238 and 5242 region,another mutation happened in T5322G and T5331G of the mtDNA COI one gene, no mutations of this gene was detected in group A and C. Serum MDA contents of group B were significantly higher than those of group A and C （P〈0.05） ,those of group C were significantly lower than those of group B and higher than group A （P〈0.05）. It can be concluded that ET could cause the mutation of mtDNA gene,and CA has antagonistic and protective effect on the mutation of mtDNA gene caused by ET.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第11期1707-1711,共5页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30560113)
云南省高端科技人才引进计划项目(2009CI125)
云南省现代农业生猪产业技术体系建设专项基金
云南省高校科技创新团队支持计划资助(云教科[2011]14号)
