摘要
目的观察甘草黄酮提取物对小肠隐窝上皮细胞IEC-6迁移及迁移过程细胞内多胺量的影响。方法划痕法制备细胞迁移模型,观察在多胺合成抑制剂二氟甲基鸟氨酸(DFMO)及钾通道抑制剂4-氨基吡啶(4-AP)存在情况下,甘草黄酮提取物对细胞迁移的影响;柱前衍生-高效液相色谱法检测IEC-6细胞在正常及DFMO存在条件下,给予甘草黄酮提取物12、24 h后细胞内多胺(精脒)的量。结果甘草黄酮提取物可逆转DFMO或4-AP所致IEC-6细胞迁移抑制;其给药12、24 h后可提高细胞内精脒的量,并逆转DFMO对多胺合成的抑制。结论甘草黄酮提取物促进细胞迁移的作用与影响细胞内多胺调控信号通路有关。
Objective To observe the effect of flavonoid from Glycyrrhizae Radix （FGR） on the migration of small intestinal epithelial cells （IEC-6） and intracellular polyamines content. Methods The cell migration model was constructed by scratch method. Under the intervention of ct-difluoromethylomithine （DFMO）, the specific inhibitor of polyamine synthesis, or 4-aminopyridine （4-AP）, the specific inhibitor of potassium channel, the effect of FGR on cell migration was observed. The intracellular polyamine content being cultured for 12 or 24 h in normal and DFMO intervention condition was detected by precolumn derivatization-HPLC. Results FGR could reverse the inhibitory effects of DFMO and 4-AP on cell migration, increase the intracellular content of polyamines after 12 or 24 h treatment, and reserve the decrease ofpolyamine content caused by DFMO. Conclusion FGR could affect the polyamine-dependent signaling pathway, which may be related to the mechanisms for FGR promoting cell migration.
出处
《中草药》
CAS
CSCD
北大核心
2013年第19期2722-2726,共5页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(30772753
81173254)
广州中医药大学中医内科学特色重点学科建设项目
关键词
甘草黄酮提取物
小肠上皮IEC-6细胞
细胞迁移
多胺
多胺调控信号通路
flavonoid from Glycyrrhizae Radix
intestinal epithelial IEC-6 cells
cell migration
polyamine
polyamine-dependent signaling pathway
