摘要
目的建立有效的体外人胚胎着床模型,为体外研究人胚胎着床过程提供条件。方法将人子宫内膜蜕膜化的间质细胞与囊胚共同培养,光镜下观察囊胚在细胞上的定位、粘附及侵入过程;免疫荧光法测定共同培养系统中的角蛋白,以确定着床模型的建立。结果与蜕膜细胞共培养5-10h后,囊胚开始黏附在细胞层上,48h后侵入蜕膜细胞间;共培养48h后,胚胎及周围的内膜细胞表达角蛋白阳性。结论囊胚与蜕膜化细胞共同培养,可以成功建立体外胚胎着床模型,更好地模拟体内着床时状态,是较理想的体外研究模型。
Objective： To eatsblish an in vitro model for embryo implantation, and provide conditions for study human embryo implantation. Methods： Human blastocysts were co - cuhured with human endometrial deeidualization cell monolayers; Time - lapse photography of implanting blastocysts; Immunostaining for cytokeratin were performed. Results： The blastoeysts adhered to the deeidualization cell layer within 5 - 10h, and Blastocysts attached to and invasion into the stromal cell layer after 48h of eoculture. Immunostaining of whole mounts of implantation sites revealed distinctive eytokeratin - positive anchoring structures adjacent to the basal surface of the trophoblast after 48h. Conclusion： Human blastocysts co - cultured with human endometrial decidualization cell monolayers could be used to study the mechanism of embryo implantation.
出处
《中国优生与遗传杂志》
2013年第10期95-96,103,F0003,共4页
Chinese Journal of Birth Health & Heredity
基金
北京市自然科学基金项目 编号:5122015
北京市科技新星基金项目 编号:H020821200190
首都医科大学附属北京妇产医院院内基金项目 编号:201114
关键词
胚胎着床
体外模型
蜕膜化
囊胚
Embryo implantation
In - vitro model
Decidualization
Blastocyst
