摘要
目的研究对甲酚（p-cresol）对人脐静脉内皮细胞（HUVEC）增殖和细胞周期的影响。方法采用CCK-8法检测HUVEC经不同浓度对甲酚作用24h、48h、72h后增殖抑制率的变化；倒置显微镜观察细胞形态学变化；流式细胞术检测对甲酚对HUVEC周期和凋亡的影响；Western印迹法检测细胞周期负调节蛋白p21的表达变化。结果对甲酚显著抑制HUVEC增殖，其抑制作用呈浓度和时间依赖性（均P〈0．05）；经对甲酚处理的HUVEC细胞形态不规则，细胞突起伸长，细胞质浑浊；流式细胞术显示经对甲酚处理的HUVEC停滞于G。期，对甲酚对HUVEC的凋亡无明显影响；对甲酚能增加HUVEC细胞p21蛋白表达，且呈剂量依赖性（P〈0．05）。结论对甲酚通过上调p21，阻滞HUVEC的细胞周期于G1期，从而抑制HUVEC增殖。
Objective To investigate the effects of p- cresol on human umbilical vein endothelial cells. Methods The effects of p- cresol on endothelial cell growth, cell cycle, cell morphological change and p21 protein were detected by the CCK- 8 assay, flow eytometry assay, inverted microscope and Western blotting. Results P- cresol could inhibit the growth of human umbilical vein endothelial cells in dose- and time- dependent manners （all P 〈 0.05）. The human umbilical vein endothelial cells treated with p- cresol became elongated processes, cloudy cytoplasm, and irregular shapes. The p-cresol stopped human umbilical vein endothelial cells at cell cycle Gl and had no effect on cell apoptosis. The p- cresol could increase protein expression of p21 in a dose dependent manner （P 〈 0.05）. Conclusion P-cresol can increase protein expression of p21, induce cell cycle arrest at Gl stage and inhibit the proliferation of human umbilical vein endothelial cells.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2013年第9期676-680,共5页
Chinese Journal of Nephrology
关键词
内皮细胞
增殖
细胞周期
对甲酚
Endothelial cells
Cell proliferation
Cell cycle
P-cresol
