摘要
目的探讨CD4^＋CD25^＋CD127^low／-调节性T淋巴细胞（Treg）在HBV感染者外周血中的表达水平及其与肝脏病理的关系。方法采用流式细胞技术检测35例HBV感染者和20名健康体检者外周血中CD4^＋CD25^＋CD127^low／-Treg的表达水平。对35例HBV感染者在B型超声引导下行肝穿刺病理检查，同时行HBV相关血清学检查。组间差异比较采用独立样本t检验，各组数据之间的相关性采用Spearman或Pearson直线相关分析。结果HBV感染者35例，ALT水平（34．57±15．36）U／L，其中ALT〈40U／L者25例，HBeAg阳性20例，HBeAg阴性15例。35例HBV感染者CD4^＋CD25^＋CD127^low／-Treg水平为（5．59±1．50）％，健康对照者为（4．85±0．95）％，两组差异有统计学意义（t=2．191，P=0．033）。HBV感染者外周血CD4^＋CD25^＋CD127^low／-Treg水平与HBVDNA载量呈正相关（r=0．495，P=0．003）。肝组织炎症活动度（G）≤2患者31例，外周血CD4^＋CD25^＋CD127^low／-Treg表达水平为（5．80±1．44）％，高于4例G〉2患者的（4．00±0．77）％，两者比较差异有统计学意义（t=2．425，P=0．021）。肝组织纤维化分期（S）〈2患者24例，外周血CD4^＋CD25^＋CD127^low／-Treg水平为（6．00±1．44）％，高于11例S≥2者的（4．70±1．24）O，两者比较差异有统计学意义（t=2．559，P=0．015）。HBV感染者外周血CD4^＋CD25^＋CD127^low／-Treg水平与炎症活动度分级呈负相关（r=-0．539，P=0．001），与纤维化分期亦呈负相关（r=一0．488，P=0．003）。结论CD4^＋CD25^＋CD127^low／-Treg与乙型肝炎的病情慢性化、肝组织损伤有关，可能在疾病的发生、发展和转归中发挥重要的调节作用。
Objective To explore the expression level of CD4^＋CD25^＋CD127low/- regulatory T cells （Treg） in the peripheral blood of hepatitis B virus （HBV）-infeeted individuals, and its association with liver pathology. Methods Thirty-five HBV-infeeted individuals and 20 healthy controls were enrolled. The frequencies of peripheral blood CD4^＋CD25^＋CD127low/- Treg were detected by flow cytometry. HBV-related serological tests as well as ultrasound-guided liver biopsies were performed in HBV-infected individuals. The two groups were compared by t test. Spearman and Pearson correlation analyses were performed. Results The alanine aminotransferase （ALT） level of HBV-infected individuals was （34. 57±15.36） U/L, with 25 subjects of ALTO40 U/L. Twenty subjects were hepatitis B e antigen （HBeAg） positive, and 15 were HBeAg negative. The frequency of the peripheral bloodCD4^＋CD25^＋CD127low/- Treg in HBV-infected individuals was （5. 59±1. 50）%, which wassignificantly higher than that in healthy controls [（4.85±0.95）%; t=2. 191, P=0. 0331. The level of peripheral blood CD4^＋CD25^＋CD127low/- Treg was positively correlated with HBV viral load （r= 0. 495, P= 0. 003）. Thirty-one subjects had liver inflammatory score ≤2, with a peripheral blood CD4^＋CD25^＋CD127low/- Treg level of （5.80±1.44）%, which was significantly higher than that of the rest 4 subjects who had liver inflammatory score 〉 2 [（4.00±0. 77）%; t= 2. 425, P = 0. 0211. Twenty-four subjects had liver fibrosis score 〈 2, with a peripheral blood CD4^＋CD25^＋CD127low/- Treg level of （6.00±1.44）%, which was significantly higher than that of the rest 11 subjects who had liver fibrosis score ≥2 [（4.70± 1.24）%; t= 2. 559, P=0. 0151. The expression of CD4^＋CD25^＋CD127low/- Treg in peripheral blood was inversely correlated with liver inflammation （r=- 0. 539, P=0.001） and fibrosis （r=-0. 488, P=0. 003） in HBV-infected individuals. Conclusions CD4^＋CD25^＋CD127low/- Treg is associated with chronic condition and liver damage of hepatitis B. It may play an important role in occurrence, development and prognosis of the disease.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2013年第10期603-607,共5页
Chinese Journal of Infectious Diseases
