摘要
目的研究铜离子对水杨酸与血清白蛋白相互作用的影响。方法采用荧光光谱技术研究了不同温度下水杨酸与牛血清白蛋白(BSA)的相互作用及铜离子对水杨酸-BSA体系的影响。结果运用理论模型处理实验数据,得到水杨酸与牛血清白蛋白(BSA)的猝灭常数、结合常数、结合力类型等相互作用参数。结论铜离子存在时,不改变水杨酸对BSA内源荧光的猝灭类型和水杨酸-BSA分子间作用的类型,但使水杨酸与BSA的表观结合常数增大。
OBJECTIVE To investigate the interactions of salicylic acid and bovine serum albumin （BSA） in the presence of Cu2＋. METHODS The interaction between salicylic acid and BSA was investigated using fluorescence at different temperatures. The effect of Cu2＋ on the salicylic acid-BSA system was also researched. RESULTS The quenching constants, binding constants and binding force type were measured according to theory model. CONCLUSION The presence of Cu2＋ doesn＇t change quenching type and force type between salicylic acid and BSA, but the apparent association constant（KLB） becomes higher.
出处
《中国现代应用药学》
CAS
CSCD
2013年第10期1066-1070,共5页
Chinese Journal of Modern Applied Pharmacy
基金
浙江省自然科学基金项目(LQ12B03001)
浙江医学高等专科学校一般研究计划项目(2013B04)
关键词
水杨酸
牛血清白蛋白
铜离子
荧光光谱法
salicylic acid
bovine serum albumin
Cu2＋
fluorescence spectrometry
