摘要
目的测定鸡骨草醇提物体外抗氧化自由基作用,为充分利用鸡骨草资源提供理论依据。方法鸡骨草饮片以65%乙醇作为溶剂,pH=3条件下浸泡24 h,通过分光光度法测定鸡骨草醇提物清除超氧自由基(·O2)、羟自由基(·OH)能力和清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基能力,普鲁士法测定其还原Fe3+能力,并以丁基羟基茴香醚(butylated hydroxylanisole,BHA)作对照测定鸡骨草醇提物对金属离子Fe2+螯合能力。结果鸡骨草醇提物具有较强的清除·O2、·OH、DPPH自由基能力,并能还原Fe3+和螯合Fe2+。结论鸡骨草醇提物具有较强抗氧化作用,其抗氧化性随着浓度的增大而增强。
OBJECTIVE To study in vitro antioxidant activity of the ethanol extracts from Abri Herba, and provide the theory to make use of Abri Herba. METHODS Abri Herba was soaked for 24 h with 65% ethanol as solvent and under the condition of pH=3, the abilities of the ethanol extracts of clearing .O2-, -OH, and DPPH were assayed by spectrophotometry. The ability of reducing Fe3＋ was detected with Prussia methodand, and chelating Fe2＋ was detected comparison to BHA. RESULTS The ethanol extracts of Abri Herba could effectively clear .O2-, .OH and DPPH, and reduce Fe3＋, chelate Fe2＋. CONCLUTION The ethanol extracts of Abri Herba antioxidation is strong, and its antioxidant activity was enhanced with the increase of concentration.
出处
《中国现代应用药学》
CAS
CSCD
2013年第10期1047-1050,共4页
Chinese Journal of Modern Applied Pharmacy
基金
国家中医药管理局"十二五"中医药重点学科建设项目(国中医药人教发[2012]32号)
广西自然科学基金资助项目(2013GXNSFAA019240)
2013年度右江民族医学院大学生创新训练计划立项项目(XJCCX201369)
关键词
鸡骨草
醇提物
抗氧化作用
清除
自由基
螯合
Abri Herba
the ethanol extracts
anti-oxidant activity
scavenging
hydroxyl radical
chelating
