摘要
目的:建立大鼠氯胺酮静脉自身给药成瘾模型。方法:成年♂SD大鼠左侧颈外静脉插管手术后,训练氯胺酮静脉自身给药,剂量为0.5 mg·kg-1,每天4 h。随后,进行5个不同剂量(0.125、0.25、0.5、0.75和1 mg·kg-1)的累进频率(progressive ratio,PR)测试。最后,进行连续10 d的自身给药行为维持,维持剂量为0.5 mg·kg-1,每天2 h。结果:90%大鼠经过7 d的训练,出现稳定的氯胺酮静脉自身给药行为;PR测试呈现明显的剂量效应;维持期内大多数大鼠仍能保持稳定的自身给药行为,2 h平均给药58次。结论:通过对大鼠自身给药行为训练期、PR测试期和维持期等阶段结果的观察与分析,说明大鼠氯胺酮自身给药模型的成功建立和维持。
To establish the animal model of ketamine self - administration in rats. Me ： Following cannulated surgery in the left external jugular vein, adult male SD rats were trained to self - administer ketamine at a dose of 0. 5 mg . kg-1 for 4 hours every day. Subsequently, the animals were tested on the progressive ratio（PR） procedure under five different ketamine doses（0. 125,0. 25, 0. 5,0. 75 and 1 mg . kg-1）. Finally,rats were maintained on fixed ratio（FR） procedure under the dose of 0. 5 mg . kg-1 for consecutive 10 days. Results： 90% of the rats acquired ketamine self - administration during 7 days of training. A significant dose - response effect was observed under the procedure of PR. A majority of the animals exhibited stable self - administration behavior on FR procedure during the maintaining phase. Conclusion： Ketamine self - administration model was successfully established in the current study.
出处
《中国药物依赖性杂志》
CAS
CSCD
2013年第5期337-341,共5页
Chinese Journal of Drug Dependence
基金
国家自然科学基金(81000573)
浙江省自然科学基金(Y2091155)
浙江省人力资源和社会保障厅"钱江人才"
关键词
氯胺酮
静脉自身给药
成瘾
动物模型
ketamine
intravenous self - administration
addiction
animal model
