摘要
目的探讨检测肺炎支原体肺炎(MPP)患儿诱导痰液中白细胞介素4(IL-4)及γ干扰素(IFN-γ)含量变化的意义。方法选取68例MPP患儿,根据疾病轻重程度分为轻症组43例和重症组25例,同时选取30名健康儿童作为对照组。采用双抗体夹心酶联免疫吸附测定法(ELISA)测定3组诱导痰中IL-4及IFN-γ含量,比较不同病期MPP患儿诱导痰中IL-4、IFN-γ的含量,以及与对照组诱导痰中IL-4、IFN-γ的含量。结果急性期患儿诱导痰IL-4含量和IL-4/IFN-γ比值显著高于恢复期患儿及对照组儿童,差异有统计学意义;急性期患儿中,轻症组诱导痰IL-4含量及IL-4/IFN-γ比值显著低于重症组,且IL-4含量显著高于恢复期轻症组;急性期重症组IL-4含量及IL-4/IFN-γ比值显著高于恢复期重症组,差异有统计学意义。结论MPP患儿存在Th1/Th2功能紊乱,且Th2反应占优势,IL-4及IFN-γ参与MPP患儿免疫状态改变,在MPP发病机制中发挥一定作用。
ABSTRACT： Objective To explore the significance of interleukin-4 （IL-4） and interferon-y （IFN-y） changes in sputum induction of children with mycoplasma pneumoniae pneumonia （MPP）. Methods Sixty-eight children with MPP were selected. According to pathological degrees, they were divided into mild group （ n = 43） and severe group （ n = 25）, meanwhile, 30 healthy children were selected as control group. Double-antibody sandwich enzyme-linked immunosorbent assay （DAS-ELISA） was applied to detect the contents of IL-4 and IFN-γ in sputum induction of 3 groups. The contents of IL-4 and IFN-γ in sputum in duction of children at different stages and of control group were compared. Results IL-4 content and IL-4/IFN-γ ratio in sputum induction of children at acute stage were significantly higher than those at recovery stage and in the control group, with significant difference. Among the children at acute stage, IL-4 content and IL-4/IFN- y ratio in mild group were markedly lower than in severe group, and the former apparently higher than in mild group at recovery stage. IL-4 content and IL-4/IFN-γ ratio in severe group at acute stage were distinctly higher than at recovery stage, and the difference showed statistical signifi- cance. Conclusion Th1/Th2 disturbance exists in children with MPP, with Th2 reaction as domi- nance. IL-4 and IFN-γ involve in immune state change in children with MPP, and play a certain role in MPP pathogenesis.
出处
《实用临床医药杂志》
CAS
2013年第17期45-47,共3页
Journal of Clinical Medicine in Practice
基金
中国高校医学期刊临床专项资金(11321093)
