摘要
以抗赤星病的净叶黄和感赤星病的NC89、长脖黄的不同烤烟品种为材料,采用盆栽试验分析成熟期不同抗性烤烟品种叶片的氮转运差异.结果表明:随着叶片的成熟,3个品种质外体NH+4的浓度均逐步增大,长脖黄、NC89质外体NH+4的浓度显著大于净叶黄;3个品种的谷氨酰胺合成酶(GS)活性均急剧下降,净叶黄的GS活性极显著大于NC89和长脖黄;3个品种的总氮含量和叶片组织NH+4浓度明显下降,长脖黄、NC89具有较高的氨气补偿点,极显著高于净叶黄.可见:不同赤星病抗性烤烟品种成熟期叶片的氮运转能力具有明显差异,抗病品种叶片的氮素再利用能力大于感病品种;感病品种以叶片质外体氨挥发形式损失的氮素多于抗病品种.
In pot culture, Jingyehuang with high resistance to tobacco brown spot and NC89, Changhohuang with high susceptibility to tobacco brown spot were selected as experimental materials, the differences of nitrogen metabolism in leaf of three flue-cured tobacco varieties with different resistance to tobacco brown spot at mature period were studied. The results indicated that, with the extending of mature period, the apoplastic NH^+4 concentration of three flue-cured tobacco varieties all increased gradually, the difference of their apoplastic NH^+4 concentration was significant, the concentration in Changbohuang and NC89 was both higher than that of Jingyehuang. Significantly dropped in glutamine synthetase activity, total-nitrogen content and leaf texture NH^+4 concentration of the three flue-cured tobacco varieties. The glutamine synthetase activity in Jingyehuang was extremely significantly higher than that of NC89 and Changbohuang. It was showed that the flue-cured tobacco variety with high resistance to tobacco brown spot had stronger reutilization capability of nitrogen than the flue-cured tobacco variety with high susceptibility to tobacco brown spot; the fluecured tobacco variety with high susceptibility to tobacco brown spot lost more nitrogen in the form of leaf apoplastic ammonia volatilization than the flue-cured tobacco variety with high resistance to tobacco brown spot.
出处
《福建农林大学学报(自然科学版)》
CSCD
北大核心
2013年第5期459-465,共7页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
贵州省烟草专卖局资助项目(中烟黔科[2012]11号)
关键词
烤烟
质外体
氮运转
烟草赤星病
flue-cured tobacco
apoplastic
ammonia translocation
tobacco brown spot