摘要
目的：探讨血清中Dickkopf（DKK）蛋白家族中的DKK-1与DKK-3蛋白在前列腺癌中的表达情况，并进一步讨论其与前列腺癌Gleason评分、骨转移等临床病理特征之间的关系。方法：应用ELISA法检测40例经前列腺穿刺活检确诊的前列腺癌患者与20例非肿瘤良性前列腺增生（benignprostatichyperplasia，BPH）患者（对照组）外周血清中DKK-1与DKK-3的表达情况，并分析相关性及与临床病理资料的关系；应用二元Logistic回归法分析前列腺特异性抗原（prostatespecificantigen，PSA）、DKK-1与DKK-3相关诊断性能，并绘制受试者工作特征曲线（receiveroperatingcharacteristiccurve，ROC）。结果：前列腺癌患者血清中DKK-1与DKK-3的表达水平均明显高于对照组患者。DKK-1水平在前列腺癌伴骨转移患者中表达水平高于不伴骨转移患者（P=0．043）；在区分骨转移方面，DKK-1的诊断效价略优于PSA（P=0．045）；DKK-3水平在高危前列腺癌患者（Gleason评分8～9）中的表达水平高于中危前列腺癌患者（Gleason评分6～7）（P=0．036）；在区分前列腺癌恶性程度上，DKK-3诊断效价优于PSA（P=0．039）。DKK-1与前列腺癌的Gleason分级无相关性，DKK-3与前列腺骨转移无相关性，并且DKK-1与DKK-3之间亦无相关性。结论：血清中DKK-1与DKK-3的表达水平与前列腺癌发生关系密切，DKK-1有可能作为诊断前列腺癌骨转移的辅助指标，而DKK-3有可能为区分前列腺癌恶性程度提供一定的依据。
Objective： To investigate the expressions of serum DKK （Dickkopf）-I and DKK-3 of Dickkopf family in patients with PC （prostate cancer） and the patients with BPH （benign prostatic hyperplasia）. To explore the correlation of their expressions with the Gleason score and bone metastasis. Methods： Serum DKK-1 and DKK-3 levels in 40 patients with prostate cancer and 20 patients with BPH were detected by ELISA （enzyme-linked immunosorbent assay） and compared by using Spearman＇s correlation analysis to evaluate the intercorrelation of DKK-1 and DKK-3 expressions and their relationship with clinicopathological characteristics. The diagnostic performance of PSA （prostate specific antigen）, DKK-1 and DKK-3 were determined by binary logistic regression method to draw the ROC （receiver operating characteristic） curves. Results： Serum DKK-1 and DKK-3 levels in patients with PC were significantly higher than those in patients with BPH. The serum DKK-1 level in PC with bone metastasis was higher than that in non-bone metastatic prostate cancer （P = 0.043）. The predictive value of DKK-1 in diagnosing bone metastasis was slightly higher than PSA （P = 0.045）. Serum DKK-3 level in high-risk PC with Gleason scores 8-9 was higher than that in moderate-risk PC with Gleason scores 6-7 （P = 0.036）. The predictive value of DKK-3 in distinguishing the grade of cancer was higher than PSA （P = 0.039）. There were no ralativeships between DKK-1 and bone metastasis, DKK-3 and Gleason grade, and DKK-1 and DKK-3. Conclusion： Serum DKK-1 and DKK-3 expressions are closely related to the occurrence of PC. DKK-1 may contribute to diagnose PC with bone metastasis. DKK-3 may support to distinguish the grade of PC.
出处
《肿瘤》
CAS
CSCD
北大核心
2013年第10期891-897,共7页
Tumor
基金
国家自然科学基金资助项目(编号:81270831)
