摘要
目的:探讨子宫腺肌病(AM)患者子宫内膜容受性下降的免疫学因素。方法:分别收集AM患者(n=18)、非AM患者(n=17)在自然周期种植窗期和控制性超促排卵周期(COH)因故放弃移植的子宫内膜组织。再将AM患者分为自然周期组(A组,n=9)和COH周期组(B组,n=9);非AM患者也分为自然周期组(C组,n=7),COH周期组(D组,n=10),采用免疫组织化学SP法检测内膜组织中CD68(巨噬细胞分子标志物)、IL-6、IL-10的表达。结果:CD68、IL-6在AM患者的内膜组织中表达较非AM患者增强(P<0.05);而IL-10的表达则相反(P<0.05);内膜组织中CD68、IL-6、IL-10的表达在A、B组间和C、D组间均无统计学差异(P>0.05)。结论:AM患者在位内膜CD68、IL-6活性增强,可能对胚胎产生毒性作用,干扰局部免疫环境,而具有妊娠保护作用的IL-10表达减低,推测三者间的共同作用是导致AM患者内膜容受性下降的免疫学因素。
Objective： To investigate the immunological mechanism of decreasing endometrial receptivity of adenomyosis （AM）. Methods： The eutopic endometrium was obtained from four groups of AM and non-AM patients during the window of implatation in natural cycle and controlled ovarian hyperstimulation （COH）. Eighteen patients of AM were divided into group A of natural cycle （n=9） and group B ofCOH （n=9）, 17 patients of non-AM were divided into group C of natural cycle （n=7） and group D of COH （n=10）. CD68, IL-6 and IL-10 were detected by immunohistochemistriy SP method. Results： The expressions of CD68 and IL-6 were stronger in the AM endometrium （groups A and B） than in the non-AM endometrium （groups C and D） with natural cycle and COH cycle （P〈0.05）. While IL-10 was expressed more weakly in the AM endometrium than in the normal endometrium of non-AM （P〈0.05）. There was no difference in the expression of CD68, IL-6 and IL-10 between groups A and B, the same between groups C and D （P〉0.05）. Conclusion： There are increasing expressions of CD68 and IL-6 in the eutopic endometrium of AM, which may have toxicity effect on the embryo and interfere the local surroundings, while IL-10 which can protect the pregnancy was down-expressed. CD68, IL- 6 and IL-10 may play an important role in the decreasing endometrium receptivity of AM.
出处
《生殖与避孕》
CAS
CSCD
2013年第10期712-717,共6页
Reproduction and Contraception
关键词
子宫腺肌病(AM)
在位内膜
免疫着床因子
adenomyosis （AM）
eutopic endometrium
immunity factors on the window of implatation
