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IL-2、IFN-α、IFN-γ对肾透明细胞癌786-0细胞中B7-H4表达的影响 被引量：2

Effect of IL-2,IFN-α and IFN-γ on expression of B7-H4 in clear cell renal cell carcinoma 786-0 cells
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摘要 目的:探究IL-2、IFN-α和IFN-γ对人肾透明细胞癌786-0细胞B7-H4表达的影响。方法:IL-2、IFN-α、IFN-γ处理786-0细胞24 h后,RT-PCR法检测B7-H4 mRNA的表达,ELISA法、免疫细胞化学法、流式细胞术检测B7-H4蛋白的表达。结果:RT-PCR结果显示,IL-2组(0.75±0.06)、IFN-α组(0.68±0.05)、IFN-γ组(0.95±0.08)786-0细胞中B7-H4 mRNA的表达均明显高于未处理组细胞(0.30±0.03)(P<0.05)。免疫细胞化学染色结果显示,于786-0细胞膜与细胞质均可检测到B7-H4蛋白表达,IL-2、IFN-α、IFN-γ处理均可增加786-0细胞B7-H4蛋白的表达。ELISA结果显示,IL-2组[(44.89±0.97)ng/ml]、IFN-α组[(46.74±2.25)ng/ml]、IFN-γ组[(47.31±1.12)ng/ml]786-0细胞上清液中分泌型B7-H4的表达明显高于未处理组[(34.42±1.69)ng/ml](P<0.05)。流式细胞术检测结果表明,IL-2组[(44.89±0.94)%]、IFN-α组[(46.41±0.55)%]、IFN-γ组[(54.18±1.42)%]786-0细胞表面B7-H4蛋白的阳性表达率明显高于未处理组[(30.45±0.96)%](P<0.05)。结论:IL-2、IFN-α、IFN-γ在转录与翻译两个环节均可上调786-0细胞B7-H4的表达水平,其中以IFN-γ上调能力最强。 Objective ： To explore the influence of IL-2, IFN-α and IFN-γ on the expression of B7-H4 in clear cell renal cell carcinoma 786-0 cells． Methods： Clear cell renal cell carcinoma 786-0 cells were stimulated by IL-2, IFN-α and IFN-γ for 24 h. The expression of B7-H4 mRNA was detected by RT-PCR. The expression of B7-H4 protein was detected by ELISA assay, cytoimmunochemistry assay and flow cytometry. Results： RT-PCR result showed that the expression of B7-H4 mRNA in IL-2 （0.75±0.06）, IFN-α （0.68±0.05） and IFN-γ （0.95±0.08） group cells was significantly higher than that in the untreated group （0.30±0.03） （P〈0.05）. Immunocytochemistry showed that the expression of B7-H4 protein was detected both in the cell membrane and cytoplasm, and the expression of B7-H4 protein was up-regulated after stimulated by IL-2, IFN-α and IFN-γ. ELISA result showed that the expression of soluble B7-H4 protein in the supernatants of IL-2 （44.89±0.97） ng/ml, IFN-α （46.74±2.25） ng/ml and IFN-γ group cells （47.31±1.12） ng/ml was significantly higher than that in the untreated group （34.42±1.69） ng/ml （P〈0.05）. Flow cytometry assay result showed that the positive expression rate of B7-H4 in IL-2 （44.89±0.94）%, IFN-α （46.41±0.55）% and IFN-γ （54.18±1.42）% group cells were significantly higher than that in the untreated group （30.45±0.96）% （P〈005）. Conclusion： IL-2, IFN-α and IFN-γ can up-regulate the expression of B7-H4, in which IFN-γ has the highest capacity.
作者 徐一鹏 朱绍兴 刘维辉 李永生 陈贵平 王华 赵阳 李方印 王宗平
机构地区 浙江省肿瘤医院泌尿外科 福建医科大学附属第二医院泌尿外科 福建医科大学附属协和医院泌尿外科
出处 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2013年第5期547-551,共5页 Chinese Journal of Cancer Biotherapy
基金 浙江省卫生适宜技术成果转化项目(No.2013ZHB001) 浙江省肿瘤医院优秀科研人才培育基金项目(No.2012YC004)~~
关键词 肾透明细胞癌 B7-H4 IL-2 IFN-Α IFN-Γ 肿瘤免疫 clear cell renal cell carcinoma B7-H4 IL-2 IFN-α IFN-γ tumor immunity
分类号 R692 [医药卫生—泌尿科学]
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中国肿瘤生物治疗杂志
2013年 第5期

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