摘要
以马传染性贫血病毒 (EIAV)驴白细胞弱毒疫苗株 (DLA)病毒基因组RNA为材料 ,用RT PCR方法扩增出EIAVgag基因 ,经平端连接将其克隆到质粒载体 pUC19中。由于疫苗不是克隆株 ,因此通过 5次单独克隆与测序 ,推导出EIAV DLA gag基因的优势序列。gag基因全长 145 8个碱基 ,编码一 486个氨基酸残基的前体蛋白。与美国EIAVWyoming 136 9株比较 ,核苷酸同源性为 80 % ,氨基酸同源性为 84 47%。其中 ,衣壳蛋白P2 6的主要同源区 (MHR)、核衣壳蛋白P11的两个CCHC型锌指结构及酸性蛋白P9的YXXL基元为高度保守的结构。为进一步研究马传染性贫血病毒弱毒疫苗的致弱机理提供了理论依据。
In order to study the attenuated mechanism of donkey leukocyte attenuated (DLA) equine infectious anemia virus (EIAV), the genome RNA of DLA EIAV was used to amplify the gag gene by RT PCR, and the PCR product was cloned to the plasmid pUC19. The cloned gag gene originated from DLA EIAV RNA was further cloned and sequenced for another five times. The resultant DLA EIAV gag gene includes 1,458 bp, which encodes a 486 amino acid precursor protein. The DLA EIAV gag shared the homology of 80% in nucleotide and 84.47% in amino acid with the EIAV Wyoming 1369 strain. Our results demonstrated that there are highly conserved structures in the major homology region (MHR) of capsid protein P26, two CCHC type zinc fingers of nucleocapsid P11 and the YXXL motif in the P9 between DLA EIAV and Wyoming 1369.
出处
《病毒学报》
CAS
CSCD
北大核心
2000年第4期361-364,共4页
Chinese Journal of Virology
基金
国家自然科学资助!项目 (39770 5 6 1)
关键词
马传染性贫血病毒
驴白细胞弱毒
核苷酸序列
equine infectious anemia virus (EIAV)
donkey leukocyte attenuated (DLA) virus
gag gene
nucleotide sequence
