摘要
研究限定枯草芽孢杆菌和乳酸杆菌固态发酵鱼汁和豆粕的混合物,转化饲料多肽。以可溶性多肽含量和胰蛋白酶抑制因子(TI)的降解率为检测指标,通过正交实验确定其最佳发酵条件为:发酵温度35℃,接种量8%,豆粕与鱼汁浓缩液(粗蛋白含量20%)质量体积比(g/mL)1∶1,发酵时间60 h。对最佳发酵条件下的产物品质进行检测,其中可溶性多肽含量达到23.54%,TI降解率为93.4%,益生菌数量达8.7×109CFU/g。通过对比发酵原料和产物,结果表明鱼汁和豆粕混合物经混合菌发酵后,其品质得到明显改善。
The process of preparing feed polypeptide from the mixture of stickwater and soybean meal fer- mented by limited mixed Bacillus subtilus and Laobacillus plantarum solid - state fermentation was stud- ied. With soluble polypeptide content and trypsin inhibitor （TI） degradation rate as detection indicators, the optimal fermentation conditions were determined by orthogonal test as follows： fermentation tempera- ture 35 ℃, inoculum size 8%, ratio of soybean meal mass to stickwater concentrated solution （ crude pro- tein content 20% ） volume 1： 1, fermentation time 60 h. Under the optimal fermentation conditions, the content of soluble polypeptide was 23.54% , the TI degradation rate was 93.4% , and the number of pro- biotics reached 8.7 x 109 CFU/g. Comparing the raw materials with fermentation products, the results showed that the quality of stickwater and soybean meal mixture fermented by mixed bacteria was greatly improved.
出处
《中国油脂》
CAS
CSCD
北大核心
2013年第10期12-15,共4页
China Oils and Fats
基金
中央级公益性科研院所基本科研业务费(2013JBFZ04)
关键词
固态发酵
鱼汁
豆粕
饲料多肽
品质分析
solid - state fermentation
stickwater
soybean meal
feed polypeptide
quality analysis
