摘要
旨在探索甘露糖结合凝集素2(Mannose-binding lectin 2,MBL2)基因与中国荷斯坦公牛精液品质及后裔生产性能的相关性,寻找可作为公牛精液品质及后裔生产性能的分子标记。利用PCR-SSCP和基因测序技术对218头成年中国荷斯坦公牛MBL2基因遗传多态性进行检测,并分析其多态位点与精液品质及后裔生产性能的相关性。结果表明,在牛MBL2基因外显子1和外显子2上存在5个突变位点,即g.101G/A、g.135C/A、g.136G/A、g.147T/C和g.32C/T,g.32C/T为新发现的多态位点(GenBank accession number:KC 832932),g.101G/A和g.135C/A突变导致了31Arg→31Gln和42Pro→42Gln突变。g.101G/A位点与精子密度呈显著相关(P<0.05);g.135C/A突变与鲜精活力及精子密度极显著相关(P<0.01);g.136G/A、g.147T/C突变与与鲜精活力、精子密度、产量育种值、脂肪育种值、蛋白育种值相关性极显著(P<0.01),与体细胞育种值显著相关(P<0.05);g.32C/T位点与体细胞育种值相关性极显著(P<0.05)。外显子1发现5种单倍型组合,MN型个体精子密度、鲜精活力、产量育种值、脂肪育种值及蛋白育种值最高,有利于提高精液品质及牛群产奶性能。结果提示,MBL2基因多态性与中国荷斯坦公牛的精液品质及后裔生产性能存在相关性。
The polymorphisms of MBL2 gene and their correlation to semen quality and progeny performance were investigated in order to find markers for semen quality and progeny perform- ance in Chinese Holstein bulls. The polymorphisms of MBL2 gene were analyzed by the method of PCR-SSCP and sequencing in 218 adult Chinese Holstein bulls. The associations between mu- tation sites of MBL2 gene and semen quality and progeny performance were analyzed. The results showed that, there were five mutations, g. 101 G/A, g. 135 C/A, g. 136 G/A and g. 147 T/C in exon 1 and a novel SNP g. 32 C/T in exon 2 （GenBank accession number： KC 832932）. The g. 101 G/A and g. 135 C/A resulted in 31 Arg→31 Gln and 42 Pro→42 Gln, respectively. The site of g. 101 G/A was significantly associated with sperm density （P〈0.05）; the g. 135 C/A was significantly associated with sperm motility and sperm density （P〈0.01）; the g. 136 G/A and g. 147 T/C SNPs had very significant effect on sperm motility, sperm density, estimated breedingvalue of milk （ EBVM）, estimated breeding value of fat （EBVF）, estimated breeding value of protein （EBVP） and estimated breeding value of somatic （EBVS） （P〈0.05） ; The g. 32 C/T SNP had very significant effect on EBVS （P〈0.01）. Five haplotype combinations were identified in exon 1 and the individuals with MN haplotype combination share the highest sperm motility, sperm density, EBVM, EBVF and EBVP to be positive for semen quality and dairy herd milk performance. The results indicate that the polymorphisms of MBL2 gene is correlated with semen quality and progeny performance in Chinese Holstein bulls.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2013年第10期1561-1568,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
"十二五"国家科技支撑计划课题(2011BAD28B02
2012BAD12B00)
江苏省农业三新工程课题(SXGC[2012]394)
