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AMHR2新型转录本AMHR2-SV1的鉴定及其在大鼠组织中的表达

AMHR2-SV1,a novel transcript of AMHR2 and its expression in rat tissues
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摘要 本研究旨在鉴定抗苗勒氏管激素受体2(AMHR2)一种新的转录本,确定其在大鼠组织中的表达情况。采用TRIzol法提取组织总RNA;运用RT-PCR和克隆测序技术分析AMHR2基因的可变剪接体及其在大鼠各组织器官中的表达差异,同时利用NCBI ORF Finder识别剪接体序列阅读框,运用Ex PASy-Prot Param和Conserved domains软件分别预测剪接体氨基酸构成及其性质和剪接体保守序列。AMHR2-SV1是AMHR2的一种新型转录本,命名为AMHR2-splice variant(AMHR2-SV1),与AMHR2参考序列比较,缺失第10外显子137 bp。利用NCBI ORF Finder和Ex PASy-Prot Param,预测AMHR2-SV1编码429个氨基酸残基,其编码的蛋白质分子量为45 990.7。Conserved domains在线软件分析表明:AMHR2-SV1蛋白序列比完整AMHR2蛋白序列缺少128个氨基酸,丝氨酸/苏氨酸蛋白激酶结构域不完整。检测结果表明,大鼠组织中均存在AMHR2和AMHR2-SV1两个转录本。在肝、脾、肺、肾、肾上腺和子宫组织中AMHR2表达量均高于AMHR2-SV1,差异达到显著水平;两种转录本在不同组织中表达趋势相同,卵巢中表达量最高,心脏中次之,其他组织表达量均显著低于卵巢和心脏。 A splice variant of anti-Mtillerian hormone receptor 2 （AMHR2）, AMHR2-splice variant （AMHR2-SV1）, was identified by RT-PCR and sequencing techniques. AMHR2-SV1 contains on open reading frame of 76-1 365 bp, encoding 429 amino acids. Sequence alignment revealed an exon 10（ 137 bp） deletion in AMHR2-SV1. Protein AMHR2-SV1 has a molecular mass of 45 990.7. Compared to the protein sequence of AMHR2, the protein sequence of AMHR2-SV1 missed 128 amino acids, and lost part of the serine/threonine kinase domain. Both AMHR2 and AMHR2-SV1 transcripts were detected in most rat tissues and organs, and the mRNA for the spliced variant was relatively less abundant than AMHR2 mRNA in liver, spleen, lung kidney, adrenal and uterus. AMHR2 and AMHR2-SV1 presented the highest expressions in ovary, the moderate expression in heart and the lowest in other tissues and organs.
作者 郭保平 牛亚茹 徐明龙 徐银学
机构地区 南京农业大学动物科学技术学院
出处 《江苏农业学报》 CSCD 北大核心 2015年第2期394-400,共7页 Jiangsu Journal of Agricultural Sciences
基金 南京农业大学科技发展基金项目(0606F0025)
关键词 AMHR2 AMHR2-SV1 新型转录本 表达图谱 anti-Miillerian hormone receptor 2（ AMHR2 ） AMHR2-SV1 novel transcript expressionprofile
分类号 Q786 [生物学—分子生物学]
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参考文献25

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江苏农业学报
2015年 第2期

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