摘要
中药草豆蔻、益智的原植物分别为山姜属草豆蔻 (AlpiniahainanensisK .Schum .)与益智 (A .oxyphyllaMiq .)。本文采用PCR直接测序法 ,首次测定了它们的核糖体DNAITS区序列 ,结果显示 ,两者序列长度 (ITS 1+ITS 2 )分别为 40 3bp与 40 4bp ,序列间具有 2 7个变异位点 (包括 5 8S编码区 )。本研究为山姜属中药材的DNA分子鉴定提供了必要的序列资料。
As the traditional Chinese medicines, Caodoukou (Semen Alpiniae Katsumadai) is from \%Alpinia hainanensis \%K. Schum., and Yizhi (Fructus Alpiniae Oxyphyllae) from \%A. oxyphylla \%Miq. Their nuclear ribosomal DNA internal transcribed spacer (ITS) regions were first sequenced. Sequence analysis showed that the ITS region (ITS 1+ITS 2) of \%A. hainanensis \%is 403 bp in length and that of \%A. oxyphylla \%is 404 bp. There are 27 variable sites (including 5.8 S coding region) in pairwise comparison of the sequences. This study could provide significant data for identifying the medicines at DNA level.
出处
《植物资源与环境学报》
CAS
CSCD
2000年第3期38-40,共3页
Journal of Plant Resources and Environment
基金
国家自然科学基金资助项目! ( 3 960 0 0 10 )
江苏省资源生物技术重点实验室资助项目
关键词
山姜属
草豆蔻
益智
核糖体DNA
ITS区序列
Alpinia Roxb.
Alpinia hainanensis K. Schum.
Alpinia oxyphylla Miq.
nrDNA
ITS sequence
