摘要
针对以对生玉米为材料的 PCR影响因素进行了系统的探讨 ,得到了适合的反应体系和循环参数。实验结果显示 ,用不同公司的 Taq DNA聚合酶扩增的 RAPD图谱不同 ,不具有重复性 ;双引物组合扩增可以检测到一些为单引物所不能检测到的位点 。
Several factors which affected RAPD reaction were studied.The results showed that the most influencing factors which affected the consistency of band patterns came from the concentration of primer,MgCl 2,dNTPs and template.A suitable system through parameter optimization was obtained.It is found that the Taq DNA polymorases from different companies performed variably.Double primer combination could detect the DNA location which could not be detected by single primer.
出处
《安徽农业大学学报》
CAS
CSCD
2000年第3期254-257,共4页
Journal of Anhui Agricultural University
基金
国家自然科学基金重大项目子课题! ( 1 9890 3 0 0 -0 1 )
关键词
RAPD
影响因素
对生玉米
分子标记
RAPD
influencing factor
opposite leaf maize
molecular marker
