摘要
目的观察益气调血,扶本培元针刺法对老年痴呆小鼠脑内p53蛋白表达的影响。方法选取SAMP8小鼠,分为SAMP8对照组、针刺组、非穴组,并设同源SAMR1对照组。针刺组采用益气调血,扶本培元针法,针刺膻中、中脘、气海、血海(双)、足三里(双);非穴组取双侧胁下两个固定非经非穴点。采用Western blot法测定小鼠皮质和海马内p53蛋白表达;HE染色法观察各组小鼠海马CA1区、颞叶、枕叶神经元细胞病理形态;免疫组化法测定皮质p53阳性细胞表达情况。结果 SAMP8皮质内p53蛋白呈较高表达的状态,针刺治疗后明显下调,与SAMP8对照组差异明显(P<0.05),与SAMR1对照组比较,差异无统计学意义(P>0.05);非穴刺激对p53蛋白表达无显著影响。海马组织内,各组p53蛋白表达差异均无统计学意义(P>0.05)。结论益气调血,扶本培元针刺法能下调小鼠脑内p53蛋白表达,改善脑细胞病理形态,从而提高老年痴呆小鼠的学习记忆能力,改善其认知功能,且具有腧穴特异性。
Objective To observe the effect of acupuncture method for benefiting qi, regulating blood, supplementing the root, and cultivating the essence （BQRBSRCE） on the p53 protein expression of mice with Alzheimer＇s disease （AD）. Methods SAMP8 mice were divided into the control group, the acupuncture group, and the non-acupoint group. The homologous SAMR1 control group was set up. Mice in the acupuncture group used acupuncture method for BQRBSRCE by needling at Tanzhong （RN17）, Zhongwan （ RN12）, Qihai （ RN6 ）, and bilateral Xuehai （ SP10 ）, and bilateral Zusanli （ ST36 ）. Two fixed non-acupoints from bilateral ribs were needled in the non-acupoint group. The p53 protein expression in the cortex and hippocampus of mice was determined using Western blot. The pathological changes of neurons in the hippocampal CA1 region, the temporal lobe, and the occipital lobe were observed using HE staining. The expression of cortical p53-positive cells was detected by immunohistochemical assay. Re- sults The p53 protein was highly expressed in the cortex of SAMPS, which was significantly down-regu- lated after acupuncture, showing statistical difference when compared with the SAMP8 control group （P 〈 0.05）, but with no statistical difference when compared with the SAMR1 control group （P 〉0.05）. Nee- dling at non-acupoints had no obvious effect on the p53 protein expression. There was no statistical difference in the p53 protein expression of the hippocampus （P 〉0.05）. Conclusion Acupuncture method for BQRBSRCE could down-regulate the p53 protein expression in the brain of mice, improve the pathological state of brain cells, thus enhancing learning and memory capabilities of AD mice, improving their cogni- tive functions, with specificity of acupoints.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2013年第10期1367-1371,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.81202739)
教育部博士点课题(No.20111210120004)
天津市教委课题(No.20080217)
关键词
针刺
老年痴呆
益气调血
扶本培元针刺法
P53蛋白
acupuncture
Alzheimer＇s disease
acupuncture method for benefiting qi, regulating blood, supplementing the root, and cultivating the essence
p53 protein
