摘要
本文建立了用核磁共振内标法快速、准确地测定苏丹红Ⅰ对照品含量的方法。采用Varian公司600 MHz核磁共振仪,在氘代氯仿为溶剂、苯甲酸为内标、25℃条件下采集苏丹红I和苯甲酸混合物的核磁共振氢谱。以苏丹红Ⅰ中化学位移在δ8.51和苯甲酸中化学位移在δ8.13的质子信号峰为定量峰,测得3批苏丹红I的质量分数分别为99.47%,99.34%和99.51%。结果表明,在没有对照品的情况下,核磁共振内标法可用于苏丹红Ⅰ对照品的含量测定和质量控制,同时也能够为其他化学对照品的含量测定提供参考。
We established a rapid and accurate method for the content determination of Sudan I with 1H-NMR spectroscopy.We acquired 1H-NMR spectra of the mixture of Sudan I and benzoic acid at 25 ℃ with Varian 600 MHz spectrometer,CDCl3 as solution,benzoic acid as internal standard.We determined the content of 99.47 ％,99.34 ％ and 99.51 ％ of 3 batches of Sudan I.Results show that the method can be applied to content determination and quality control of Sudan I.
出处
《山东科学》
CAS
2013年第5期10-13,共4页
Shandong Science
基金
国家质检公益性行业科研专项(201110248-6)
关键词
核磁共振氢谱
含量测定
苏丹红Ⅰ
1H-NMR spectroscopy
content determination
Sundan I
