摘要
目的 :为探讨幽门螺杆菌 (Helicobacterpylori ,Hp)的感染方式和感染途径 ,对口腔卫生和胃病关系进行了调查 ,并对不同使用时间和不同保管方法的牙刷上Hp的分布进行了研究。方法 :对 2 94 5名中老年人进行口腔卫生和胃病调查 ;并从随机抽取的 119支使用中的牙刷进行标本采集 ;采用PCR技术对从中提取Hp的染色体DNA扩增 ,检测Hp。结果 :口腔卫生情况中优、良、中、差 4个组的胃病发生率分别为 5.98%、16.84 %、4 4 .83%和 50 .78% ,有较为明显的差异 (P <0 .0 5) ;不同使用时间 ( <15d ,15～ 30d ,30～ 60d ,>60d)牙刷毛上的Hp检测阳性率分别为 3.57%、10 .0 0 %、2 0 .61%和 4 6.88% ,显示使用时间越长 ,Hp在牙刷上存在的可能性越大 (P <0 .0 5) ;牙刷保存于干燥环境中 ,其Hp阳性率明显低于潮湿环境中 (P <0 .0 1)。结论 :口腔和牙刷可能是Hp的一条传播通道 。
Objective:To investigate the relationship of oral hygiene and distribution of Helicobacter pylori(Hp) to stomach diseases. Methods: 2945 cases were studied on their oral hygiene and stomach disease. The deposit and food debris collected from 119 toothbrushes were detected by polymerase chain reaction for the presence of Hp. Results:The subjects were divided into 4 groups according to their oral hygiene, and the incidence of stomach disease for each qroup was 5.98%,16.84%,44.83% and 50.78% respectively (P<0.05).The toothbrushes were also divided into four groups according to their using duration. The positive rates of Hp at different using times(<15 d,15～30 d,30～60 d,>60 d) were 3.57%,10.0%,20.61% and 46.88%(P<0.05) respectively.The method to keep the toothbrush was related to Hp positive rate too,dry place was better than wet place(P<0.01). Conclusion: The results of Hp detection in toothbrush suggested that Hp colonization is not restricted in the gastric mucous and this ecological niche may serve as a possible sanctuary which may responsible for resettlement of the stomach Hp. Oral hygiene, toothbrush keeping method and using duration are also very important to stomach health.
出处
《解放军预防医学杂志》
CAS
北大核心
2000年第6期415-418,共4页
Journal of Preventive Medicine of Chinese People's Liberation Army
关键词
口腔卫生
牙刷
幽门螺杆菌
胃疾病
聚合酶链反应
oral hygiene, toothbrush, stomach disease, polymerase chain reaction(PCR), Helicobacter pylori(Hp)
