摘要
目的 :为研究有机磷化合物的细胞毒性及其在缺氧或低氧环境中毒性升高的机理 ,为高原有机磷中毒的预防和治疗用药提供依据。方法 :利用MTT比色法、放免技术及荧光分析法测定缺氧条件下梭曼中毒PC12细胞存活率、cAMP含量和细胞内游离钙浓度 (〔Ca2 +〕i)的变化。结果 :单纯梭曼中毒组PC12细胞存活率无明显改变 ,缺氧及缺氧中毒组细胞存活率显著下降 ;缺氧组、梭曼中毒组及缺氧梭曼中毒组的细胞cAMP含量〔Ca2 +〕i水平均明显升高 ,且缺氧中毒组的变化较单纯缺氧、梭曼中毒组更为显著 (P <0 .0 1)。结论 :缺氧导致细胞信号传导增强 ,加重神经性毒剂的细胞损伤 。
Objective:To study the cytotoxicity of soman during hypoxia on PC12 cells and the mechanism of increasing toxicity of organophosphate compounds under the condition of hypoxia.Methods:The cytotoxicity was measured by MTT spectrophotometric method,the concentration of cAMP and 〔Ca 2+ 〕i were detected with RIA and fluorescent probe (Fura 2/AM) and protein content was assayed by Folin phenol method.Results:Both hypoxia andsoman intoxication with hypoxia remarkably reduced the survival rate of PC12 cells,but in soman intoxication alone no obvious change of survival rate was found;the concentration of cAMP and〔Ca 2+ 〕i in PC12 cells were remarkably increased in hypoxia, soman intoxication and soman intoxication with hypoxia groups. The changes in soman intoxication with hypoxia group were more significant(p<0.01).Conclusion:The increase of signal transduction and the exacerbation of neurotoxicity on PC12 cells induced by hypoxia may play an important role in the increase of toxicity of organophosphate compounds during hypoxia.
出处
《解放军预防医学杂志》
CAS
北大核心
2000年第6期400-402,共3页
Journal of Preventive Medicine of Chinese People's Liberation Army
