摘要
目的 观察三苯氧胺对人正常皮肤与烧伤后增生性瘢痕来源的成纤维细胞的 DNA及胶原合成的抑制作用 ,探讨临床应用三苯氧胺治疗烧伤后增生性瘢痕的可行性 .方法 体外分离及培养正常皮肤及烧伤后增生性瘢痕组织内的成纤维细胞 ;采用 3H- Td R掺入和脯氨酸比色法 ,比较两种成纤维细胞经不同浓度三苯氧胺作用后 DNA及胶原合成的变化 .结果 三苯氧胺可抑制正常皮肤及烧伤后增生性瘢痕成纤维细胞的 DNA及胶原合成 ,但对两种不同来源的成纤维细胞的抑制率显示明显的差异 .相同浓度的三苯氧胺对增生性瘢痕成纤维细胞的抑制幅度显著大于正常皮肤成纤维细胞 .三苯氧胺使正常皮肤和增生性瘢痕成纤维细胞的 5 0 % DNA合成速率受抑制的浓度分别为 (19.2± 0 .7) μmol· L- 1 和 (8.3±0 .3) μmol·L- 1 (P<0 .0 1) ;使 5 0 %胶原合成受抑制的浓度分别是 (2 5 .3± 0 .2 )μm ol· L- 1 和 (8.5± 0 .3)μmol· L- 1 (P<0 .0 1) .结论 三苯氧胺能有效地抑制体外培养的皮肤成纤维细胞的生长增殖及胶原合成 ;与正常皮肤成纤维细胞比较 ,烧伤后增生性瘢痕成纤维细胞对三苯氧胺的抑制作用更为敏感 ;
AIM To investigate the effect of tamoxifen on DNA and collagen syntheses of human normal skin fibroblasts and hypertrophic scar fibroblasts. METHODS Human normal skin- and hypertrophic scar-derived fibroblasts were cultured in media containing serial dilutions of tamoxifen. DNA and collagen syntheses of the fibroblasts were determined by 3H-TdR incorporation and hydroxy proline colorimetric analysis. RESUTLS DNA and collagen syntheses were inhibited dose-dependently with tamoxifen. The 50% DNA synthesis rate was reduced with tamoxifen of (19.2±0.7) μmol·L -1 acting on human normal skin-derived fibroblasts and (8.3±0.3) μmol·L -1 on hypertrophic scar-derived fibroblasts. The 50% collagen synthesis was inhibited with tamoxifen of (25.3±0.2) μmol·L -1 on human normal skin-derived fibroblasts and (8.5±0.3) μmol·L -1 on hypertrophic scar-derived fibroblasts respectively. CONCLUSION Tamoxifen could inhibit DNA and collagen syntheses of both human normal skin and hypertrophic scar-derived fibroblasts, but preferred to influence hypertrophic scar-derived fibroblasts more effectively. This might indicate that in vivo tamoxifen could selectively act on the fibroblasts, which shows the phenotypic characteristics of hypertrophic scar fibroblasts.
出处
《第四军医大学学报》
2000年第12期1527-1529,共3页
Journal of the Fourth Military Medical University
