摘要
目的:探索新生大鼠海马神经前体细胞培养方法,并观察低浓度丙泊酚对体外培养海马神经前体细胞增殖的影响。方法:无菌获取新生大鼠海马神经前体细胞进行体外培养,利用免疫组化的方法对神经前体细胞特性进行鉴定。并采用MTT法和3H-TdR掺入法观察丙泊酚对海马神经前体细胞增殖的影响。结果:从新生大鼠海马分离得到的细胞接种于含有表皮生长因子和碱性成纤维细胞生长因子两种丝裂原刺激因子的条件培养基中,可以持续分裂增殖并形成细胞克隆(神经球)。该神经球可以表达神经上皮干细胞蛋白(巢蛋白),同时也可以探测到外源性给予的细胞增殖特异性标记物(B rdU)。与脂肪乳对照相比,低浓度的丙泊酚(0.5μmol.L-1、2.5μmol.L-1)处理组A570nm值升高(P<0.05)和3H-TdR掺入值增加(P<0.01)。结论:用此方法获取并培养的海马细胞具有神经前体细胞的特性。低浓度的丙泊酚可以促进体外培养的海马神经前体细胞增殖。
Objective：To investigate a practical method to culture the neural precursor cells from hippocampus of neonatal Wester rats, and to study the effects of propofol on its proliferation. Methods： Hippocampus precursor cells cultured in vitro were identified with immunocytochemistry methods. Meanwhile, MTT method and ^3 H-TdR absorption were detected to determine the propofol effects on the precursor cells＇ proliferation. Results：With the presence of epidermal growth factor （EGF） and basic fibroblast growth factor （bFGF） in the medium, the cells isolated from the newborn rats＇hippocampus could continuously proliferate and form into cell clones （neurospheres）. Furthermore, the neurospheres could express the neuroepithelial stem cell protein （nidogen） , and bromodeoxyoridine （ BrdU）, which given exogenously, could be tracked in the neurospheres. Contrasting to the intralipid group, the A570nm value and the ^3H-TdR absorption increased with the treatment of low concentration propofol （0.5 μmol · L^-1 , 2.5 μmol·L^-1 ）. Condttsion ： The cells obtained and cultured by this method process the property of the neural precursor cells. Low concentration of propofol can enhance the proliferation of hippocampus neural precursor cells.
出处
《军医进修学院学报》
CAS
北大核心
2007年第1期56-58,共3页
Academic Journal of Pla Postgraduate Medical School
基金
国家自然科学基金项目(30571791)
关键词
海马
神经前体细胞
二异丙酚
hippocampus
neural precursor cell
propofol
