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磷酸化p38在高温处理后金黄地鼠胚心脏的表达

Expression of phosphorylated p38 in embryonic heart of hamster after hyperthermia
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摘要 目的研究心脏正常发育和异常分化过程中磷酸化p38(p-p38)的表达变化,探讨p38在高温处理后金黄地鼠胚胎心脏发育中的作用。方法将金黄地鼠孕鼠于受孕8d置42℃水浴持续20min,于高温处理后12h、24h、36h、48h剖腹取胎,制备石蜡切片。利用免疫荧光染色技术,观察心脏正常发育和异常分化过程中磷酸化p38(p-p38)的表达变化。结果 p-p38的免疫阳性产物表达于鼠胚内皮细胞及心胶质细胞胞浆中,高温处理后不同时间点实验组p-p38的表达均较对照组明显降低。结论高温可导致金黄地鼠胚胎心脏p-P38表达减弱,p-p38对心脏的正常发育具有重要作用。 Objective To study the expression of p-p38 protein in the embryonic heart and explore the effect of hyperthermia on the development of hamster embryonic heart. Methods Hamsters were subjected to hyperthermia （in 42℃ hot water for 20 min） in embryonic 8 days. The embryos were obtained at 12h, 24h, 36h, 48h after treatment respectively, and serial sections of embryonic heart were prepared. The expression of p-p38 protein in the embryonic heart was observed with immunofluoreseent staining technique. Results The p-p38 immunoreactive product was distributed diffusely in the cytoplasm, the staining intensity of p-p38 immunoreactive product in the cardiac endothelial cells and jelly was decreased in all experimental hyperthermia groups than in control group. Conclusion The decreased expression of p-p38 in the embryonic heart under hyperthennia indicates that p-p38 may play an important role in the normal development of hamster heart.
作者 冷昭廷 刘文静 闫雪 王力 付志达 杨磊 于丽 隽兆东
机构地区 潍坊医学院外科学教研室 潍坊医学院组织学与胚胎学教研室
出处 《解剖科学进展》 CAS 2013年第5期407-409,共3页 Progress of Anatomical Sciences
基金 国家自然科学基金资助(No.30900775) 山东省自然科学基金资助(ZR2010CQ036)
关键词 心脏 高温 P-P38 胚胎 金黄地鼠 heart hyperthermia p-p38 embyo hamster
分类号 R331.31 [医药卫生—人体生理学]
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参考文献14

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