摘要
【目的】建立sYP-9080原药高效液相色谱分析方法。【方法】采用AgilentZORBAXSB—C18色谱柱，流动相为乙腈-水（体积比45：55），流速为1．0mL／min，紫外检测波长254nm条件下进行外标法定量。【结果】该方法300-700mg／L质量浓度范嗣内呈良好线性，回收率在99．50％-100．01％之间，标准偏差为0．13，变异系数为0．14％。【结论】该方法操作简便，准确度和精密度高，线性相关性好。
[Aims] A HPLC method for analysing SYP-9080 was established. [Methods] The sample was separated and analyzed quantitatively on Agilent ZORBAX SB-C18 reversed-phase chromatography column and UV detector at 254 nm, using acetonitrile and water （45 ： 55, by vol） as the mobile phase. The rate of flow was 1.0 mL/min. [Results] Good linearity was obtained in range from 300-700 mg/L. The recovery rate was 99.50-100.01%. The results showed that the standard deviation was 0.13. The variation coefficient was 0.14%. [Conclusions] The method was simple, rapid and accurate, and had good linear correlation.
出处
《农药》
CAS
CSCD
北大核心
2013年第10期734-735,共2页
Agrochemicals
