摘要
【目的】明确新型杀虫剂氯虫苯甲酰胺在植株内的内吸传导特性,为合理使用氯虫苯甲酰胺防治蔬菜、水稻等害虫策略的制定提供科学依据。【方法】采用水培法和涂药法分别研究氯虫苯甲酰胺在大豆植株中是否具有内吸传导特性。以乙腈为溶剂,超声波处理提取大豆植株中的氯虫苯甲酰胺,经预净化,由高效液相色谱(带二极管阵列检测器)检测分析氯虫苯甲酰胺在大豆植株中各部位的分布状况。【结果】采用水培法处理,培养液中氯虫苯甲酰胺的浓度为50μg.mL-1时,24 h后大豆茎杆和叶片中的含量分别为15.22和4.73μg.g-1,48 h后大豆茎杆和叶片中的含量分别为8.71和7.96μg.g-1;当培养液中氯虫苯甲酰胺的浓度为200μg.mL-1时,24 h后大豆茎杆和叶片中的含量分别为18.52和11.95μg.g-1,48 h后大豆茎杆和叶片中的含量分别为16.45和17.88μg.g-1。采用浓度100μg.mL-1药液涂药处理大豆中部成熟叶片时,24和48 h后检测发现处理叶片以上部位的叶片中氯虫苯甲酰胺的含量分别为16.55和20.79μg.g-1,处理叶片以下部位未检测出氯虫苯甲酰胺;在大豆顶端生长点叶片涂抹浓度100μg.mL-1药液时,在生长点以下部位的叶片中均未检测出氯虫苯甲酰胺。【结论】研究结果表明氯虫苯甲酰胺在大豆植株内具有优异的自下而上的内吸传导特性。
【Objective】The objective of this study is to investigate intake transportation function of new insecticide chlorantraniliprole and to provide evidences for formulating strategies for controlling vegetable pests and rice pests in the present study.【Method】 Intake transportation function of chlorantraniliprole was investigated through applicating chlorantraniliprole on the soybean leaves or water culture.The acetonitrile was used as extracted solven,and chlorantraniliprole in the soybean was extracted by using the ultrasonic extracting method and analyzed by high performance liquid chromatography（with diode-array detector）.【Result】When soybean roots were incubated with chlorantraniliprole solution at a concentration of 50 μg·mL-1,the concentrations of chlorantraniliprole in soybean stems and soybean leaves were 15.22 and 4.73 μg·g-1 after treatment for 24 h,respectively.The concentrations of chlorantraniliprole in soybean stems and soybean leaves were 8.71 and 7.96 μg·g-1 after treatment for 48 h,respectively.When soybean roots were incubated with chlorantraniliprole solution at a concentration of 200 μg·mL-1,the concentrations of chlorantraniliprole in soybean stems and soybean leaves were 18.52 and 11.95 μg·g-1 after treatment for 24 h,respectively.The concentrations of chlorantraniliprole in soybean stems and soybean leaves were 16.45 and 17.88 μg·g-1 after treatment for 48 h,respectively.When chlorantraniliprole was applied on the mature leaves at a concentration of 100 μg·mL-1,chlorantraniliprole was found in the apical leaves and its concentration was 16.55 and 20.79 μg·g-1 after treatment for 24 and 48 h,respectively,while no chlorantraniliprole was detected in the leaves below the mature leaves.When chlorantraniliprole was applied on the apical leaves at a concentration of 100 μg·mL-1,while no chlorantraniliprole was detected in the leaves below the apical leaves.【Conclusion】 The results indicated that chlorantraniliprole had good intake transportation function in soybean and which could transport from the bottom to up of the plant.
出处
《中国农业科学》
CAS
CSCD
北大核心
2011年第11期2276-2283,共8页
Scientia Agricultura Sinica
基金
江苏省高校自然科学基金(10KJB210006)
扬州大学高层次人才引进基金(2009)
扬州大学博士后科研基金(2009-2011)
关键词
氯虫苯甲酰胺
大豆
内吸
传导
chlorantraniliprole
soybean
intake
transportation
