摘要
目的：观察扭曲蛋白（Twist）、信号转导和转录激活因子3（STAT3）、上皮钙黏素（E—cad）在胃癌组织及癌旁组织中的表达，探讨三者与胃癌临床病理参数之间的相关性及三者的表达对判断胃癌预后的意义。方法：采用免疫组织化学方法链霉亲和素一生物素复合物（SABC）法检测手术切除胃癌组织及癌旁组织标本中的Twist、STAT3、E—cad表达，分析其与胃癌患者年龄、性别、肿瘤的组织学类型、转移及TNM分期等参数的关系。结果：在胃癌组织中Twist、STAT3、E．cad的阳性率分别为57．3％、64．7％、12．0％，癌旁组织中的阳性率分别为17．3％、10．8％和68．0％，差异均有统计学意义（P〈0．01）。Twist的表达在肿瘤远处转移、淋巴结转移、TNM分期和浸润深度间差异均有统计学意义（P〈0．05-P〈0．01）；STAT3的表达在肿瘤淋巴结转移、浸润深度及TNM分期间差异均有统计学意义（P〈0．05～P〈0．01）。Twist、STAT3表达与E—cad表达均呈明显负相关关系（P〈0．01）。结论：胃癌组织中Twist、SATA3表达与肿瘤的生物学特征均有一定关系，SATA3的激活可能会增强Twist、E—cad的表达，检测Twist、STAT3、E—cad对预测肿瘤侵袭转移、判断预后具有一定价值。
Objective： To investigate the expressions of Twist protein （Twist）, signal transducer and activator of transcription 3 （STAT3）, epithelial mesenchymal transition related factor （E-cad） in gastric cancer tissue and peritumoral tissue, analyze their correlations and explore their significance in judging the prognosis of patients with gastric cancer. Methods：The expressions of Twist, STAT3 and E-cad in gastric cancer tissue and peritumoral tissue were detected by immunohistochemistry streptavidin-biotin complex （SABC） , their relationships with age, gender, histological type of tumor, metastasis and TNM stage of patient were analysed. Results： The positive rate of Twist, STAT3 and E-cad in gastric cancer tissue were 57.3% ,64.7% and 12.0% ,respectively. The positive rate of Twist, STAT3 and E-cad in peritumoral tissue were 17.3% , 10.8% and 68.0% ,the expression differences of which were not statistical significanee（P 〈 0.01 ）. The differences of Twist expression in different tumor metastasis, lymph node metastasis, TNM staging and depth of invasion were statistical significance （ P 〈 0.05 - P 〈 O. 01 ）. There was statistically significant in the expression of STAT3 between node metastasis,depth of invasion and TNM stage（P 〈0.05 -P 〈0.01 ）. They were negative correlation in Twist and STAT3 expressions and E-cad expression（P 〈 0.01 ）. Conclusions：The expressions of Twist and SATA in gastric cancer tissue have a certain relationship with tumor biological characteristics, the expression of SATA3 may activate the expression of Twist and E-cad. The detection of Twist,STAT3 and E-cad can predict the tumor invasion,metastasis and prognosis of patient.
出处
《蚌埠医学院学报》
CAS
2013年第10期1234-1237,共4页
Journal of Bengbu Medical College
基金
安徽省高校省级自然科学研究项目(KJ2012B210)
关键词
胃肿瘤
信号转导和转录激活因子3
扭曲蛋白
上皮间质转化
gastric carcinoma
signal transducers and activators of transcription 3
twist protein
epithelial mesenchymal transition
