摘要
目的:分别通过气道内注入内毒素、单独香烟烟熏以及烟熏联合内毒素3种方法构建慢性阻塞性肺病(COPD)大鼠模型,寻找经济、便捷、成功率高的COPD建模方法。方法:将雄性SD大鼠随机分为健康对照组、烟熏组、内毒素组和烟熏+内毒素组,每组10只。90 d后HE染色观察大鼠肺、支气管的病理改变。酶联免疫吸附试验(ELISA)检测各组大鼠血清及肺组织匀浆中白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)、磷脂酶A2(PLA2)含量的变化。结果:烟熏+内毒素组大鼠肺、支气管的HE染色符合COPD的病理改变;其血清及肺组织中IL-8、TNF-α、PLA2含量较其他3组明显增多,差异均有统计学意义(P<0.05)。结论:烟熏加气管内注入内毒素法用于建立大鼠COPD模型优于单独烟熏法及单独气道内注入内毒素法。
Objective：To develop an economical,convenient and effective approach to establish chronic obstructive pulmonary disease（COPD） rat model using lipopolysaccharide（LPS） infusion of the airway,cigarette exposure alone,and cigarette exposure joined with LPS infusion of the airway,respectively. Methods： The male Sprague-Dawley（SD） rats were randomly assigned into the healthy control,smoke exposure,LPS,and smoke plus LPS groups with 10 rats in each group. Ninety days later, we used hemotoxylin-eosin （HE） staining to investigate the pathological changes in the lung and bronchus,and ELISA to measure the changes in the level of interleukin-8（IL-8）,tumor necrosis factor-α（TNF-α）,and phospholipase A2（PLA2） in the serum and lung tissue homogenates. Results：In the smoke-LPS group,HE staining revealed that the pathological changes of the lung and bronchus were similar to those observed in human COPD. There was a remarkable increase of IL-8,TNF-α,and PLA2 in serum and pulmonary tissues as compared to the other three groups,with a statistically significant difference（P 〈 0.05）. Conclusion：COPD rat model established by the airway infused with LPS and fumed by cigarettes is proved to be successful and better than the two other approaches.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第9期1226-1230,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
南京市卫生局科技发展计划(YKK10103)
关键词
慢性阻塞性肺病
大鼠
内毒素
香烟烟熏
chronic obstructive pulmonary disease
rat
lipopolysaccharide
smoke exposure
