摘要
旨在利用实时荧光定量PCR和Western blot分别研究新疆一枝蒿提取物黄酮及萜类对棉铃虫P450 CYP6B6在mRNA水平和蛋白水平上表达的影响。实时荧光定量PCR结果表明,P450 CYP6B6在mRNA水平的表达量受到诱导。时间效应为P450 CYP6B6在处理的12 h时能够被黄酮和萜类提取物显著诱导表达,并且在24 h时表达受到抑制;浓度效应为处理24 h时,黄酮和萜类提取物浓度分别为20 mg/100 g和1 mg/100 g时显著诱导P450 CYP6B6的表达,并且在40 mg/100 g和2 mg/100 g时抑制P450 CYP6B6的表达;Western blot检测结果表明,萜类和黄酮在时间和浓度效应上都能显著诱导P450 CYP6B6蛋白表达,并且蛋白水平在时间与浓度效应中的表达趋势与mRNA水平相似。
In order to investigate the cytochrome F450 CYP6B6 in midgut induced by flavones and terpenes extracts from Artemisia rupestris, we detected the CYP6B6 expression in mRNA level by Real-time quantitative PCR （ qPCR ） , and at protein level by Western blot. qPCR results showed that the 17450 CYP6B6 mRNA levels in the midgut of the larvae were induced by these two extracts. For the time effect, when the larvae were exposed to 20 mg/lO0 g flavones extracts and 2 mg/lO0 g terpenes extracts respectively for 12 h the relative expression level of P450 CYP6B6 mRNA in the midgut was over-expressed, while at 24 h the expression was inhibited. For the concentration effect, when the larvae were treated with these two exyracts for 24 h, the relative expression level of P450 CYP6B6 mRNA was over-expressed by 20 mg/lO0 g flavones and 1 mg/100 g terpenes extracts, respectively, while the expression was inhibited by 40 mg/lO0 g flavones and 2 mg/100 g terpenes extrcts in the diet, respectively.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第9期130-135,共6页
Biotechnology Bulletin
基金
国家自然科学基金项目(31260444)
新疆维吾尔自治区自然基金项目(2012211A018)
