摘要
作为发展可食性疫苗的第一步,将猪源布鲁氏菌蛋白OMP31的编码基因转入番茄,获得了转基因番茄。利用不依赖于连接反应的克隆方法(ligation-independent cloning,LIC)构建了pJG045-Omp31植物表达载体,将此表达载体通过三亲融合法转入农杆菌AGL-0中,用农杆菌介导的植物叶盘转化法转化番茄,并通过对再生植株的基因组中OMP31的编码基因扩增测序,最终筛选出5株转基因番茄。
Transgenic tomato carrying Brucella suismembrane protein OMP31 gene was constructed for the development of edible vaccine.In the experiment,the Omp31 gene was inserted into a plant expression vector pJG045 using ligation-independent cloning or LIC;the recombinant plasmid was transferred intoAgrobacterium tumefaciensAGL-0 by triparental cross;then the transformed Agrobacteriumwas used to infect tomato cotyledons discs,and transgenic seedlings were identified by antibiotics resistance screening and PCR;5 transgenic tomato plants were obtained.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第9期119-123,共5页
Biotechnology Bulletin
基金
内蒙古自治区自然科学基金项目(00511702)
