期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

布鲁菌OMP25基因重组山羊痘病毒的构建 被引量：3

Construction of the Recombinant Goatpoxvirus Expressing OMP25 Gene of B.melitensis
在线阅读 下载PDF
导出
摘要 将羊种布鲁菌外膜蛋白25(OMP25)基因插入到山羊痘病毒转移载体PGM-TK-I1L-GPT1启动子I1L的下游,构建重组山羊痘病毒转移载体。用脂质体转染法将重组转移载体与山羊痘病毒疫苗株G14-STV44-55共转染绵羊成纤维细胞,在细胞内同源重组。通过霉酚酸药物筛选、纯化,经PCR鉴定获得了重组山羊痘病毒。 To construct the Recombinant goatpoxvirus expressing keywrod＇s ontside membrance protein （OMP25） gene of B. melitensis, the OMP25 gene was inserted into the downstream of promotor IlL of goatpoxvirus（GPV）transfer vector PGM-TK-IIL-GPT1. Then the recombinant transfer vector was transfected into sheep fibroblast ceils infected with GPV vaccine strain G14-STV44-55 by lipofectamine. The transfected sheep fibroblast ceils were conducted by mycophenolic acid（MPA）to screen and purify recombinant goatpoxvirus（RGPV）. Recombinant goatpoxvirus was successfully obtained and identified by PCR.
作者 魏凤 郭志儒 陈创夫 乔军
机构地区 石河子大学动物科技学院
出处 《动物医学进展》 CSCD 2008年第4期6-9,共4页 Progress In Veterinary Medicine
基金 新疆生产建设兵团博士基金(30560109)
关键词 羊种布鲁菌 外膜蛋白25基因 重组山羊痘病毒 B. rnelitensis outside membrance protein 25 gene recombinant Goatpoxvirus
分类号 Q784 [生物学—分子生物学] S852.614 [农业科学—基础兽医学]
  • 相关文献

参考文献10

  • 1李凡卡,卿燕,任德坤,熊军.新疆生产建设兵团布鲁氏菌病疫情现状与防制对策[J].地方病通报,2000,15(1):64-65. 被引量：3
  • 2Mackett M,Smith G L,Moss B. Vaccine virus,A selectable eukaryotic cloning and expression vector[J]. Proc Natl Acad Sci USA, 1982,79 : 7415-7419.
  • 3Diallo A, Minet C, Berhe G. Goat immune response to capripox vaccine expressing the hemagglutinin protein of peste des petits ruminants[J]. Ann N Y Acad Sci,2002,969:88-91.
  • 4Romero C H, Barrett T, Kitching R P. Protection of goats against peste des petits ruminants with recombinant capripoxviruses expressing the fusion and haemagglutinin protein genes of rinderpest virus[J]. Vaccine, 1995, 12:136-140.
  • 5Wade-Evans A M, Romero C H, Mellor P. Expression of the major core structural protein(VPT) of bluetongue virus, by a recombinant capripox virus, provides partial protection of sheep against a virulent heterotypic bluetongue virus challenge[J]. Virology, 1996, (1):227-231.
  • 6Berhe G, Minet C, Le Goff C. Development of a dual recombinant vaccine to protect small ruminants against peste-des-petitsruminants virus and capripoxvirus infections[J]. Virology, 2003, (2):1571-1577.
  • 7乔传玲,于康震,邓国华,王秀荣,孟庆文,田国斌,唐秀英.禽流感病毒A/Goose/Guangdong/3/96(H5N1)NA基因的克隆及其重组禽痘病毒转移载体的构建[J].中国兽医学报,2003,23(2):111-114. 被引量：9
  • 8Taylor J, Edbauer C, Rey Senelonge A, et al. Newcastle disease virus fusion protein expressed in a fowlpox virus recombinant confers protection in chickens[-J]. Virology, 1990, 64(4),1441-1450.
  • 9周建胜,马慧玲,郭庆山.绵羊睾丸原代细胞培养及接种羊痘弱毒后的病变观察[J].中国兽医科技,2004,34(9):71-74. 被引量：8
  • 10Falko G, Bernardmoss F. Transient dominant selection of recombinant vaccinia viruses [J ]. J Virol, 1990, 64 ( 6), 3108- 3111.

二级参考文献6

共引文献17

同被引文献20

  • 1杨春华,邱昌庆,曹小安.流产布鲁氏菌omp25基因毕赤酵母表达载体的构建和鉴定[J].中国人兽共患病学报,2007,23(7):693-695. 被引量：3
  • 2Panicali D, Paoletti E. Construction of poxviruses as cloning vectors:insertion of the thymidine kinase from herpes simplex virus into the DNA of infectious vaccinia virus[J]. Proc Nat Acad Sci USA,1982,79(16) :4927-4931.
  • 3Perrin A,Aibina E,Breard E,et al. Recombinant capripoxviruses expressing proteins of bluetongue virus: evaluation of immune responses and protection in small ruminants[J]. Vaccine, 2007,25 (37): 6774-6783.
  • 4Wallace D B, Weyer J, Nel L H, et al. Improved method for the generation and selection of homogeneous lumpy skin disease virus (SA-Neethling) recombinants[J]. J Virol Methods, 2007,146 ( 1 ) : 52-60.
  • 5Wallace D B,Viljoen G J. Immune responses to recombinants of the South African vaccine strain of lumpy skin disease virus generated by using thymidine kinase gene insertion[J]. Vaccine, 2005,23 (23) : 3061-3067.
  • 6斯佩克特 D L,戈德曼 R D.细胞实验指南[M].3版.黄培堂,王嘉玺,朱厚础,等译.北京:科学出版社,2001:1-32.
  • 7Falkner P G, Moss B, Escherichia coli gpt gene provides dominant selection for Vaccinia virus open reading frame expression vectors[J]. J Virol, 1988,62 (6) : 1849-1854.
  • 8Kate A,Jo-Ann P, Friedrich T, et al. Evaluation of lumpy skin disease virus, a capripoxvirus, as a replication-deficient vaccine vector[J]. J Gen Virol, 2003, 84(3) : 1985-1996.
  • 9Shawn B, Geoff P, John C, et al. Evaluation of an ovine testis cell line (OA3. Ts) for propagation of capripoxvirus isolates and development of an immunostaining technique for viral plaque visualization[J]. J Vet Diagn Invest,2007,19(5):486-491.
  • 10Mulligan R C, Berg P. Selection for animal cells That express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase [J]. Proc Natl Acas Sci USA,1981,78(4) :2072-2076.

引证文献3

二级引证文献1

动物医学进展
2008年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部