摘要
旨在探索草莓玻璃化试管苗恢复措施。以‘丰香’草莓玻璃化试管苗为材料,采用正交设计的方法,在继代培养基中添加不同浓度组合的活性炭、聚乙烯醇和钙离子(氯化钙),研究不同组合对草莓玻璃化试管苗恢复的影响,同时比较恢复后的正常试管苗与原来的玻璃化苗及正常苗的生理生化指标。结果表明,正交设计的9种处理间草莓玻璃化苗的恢复率差异显著,恢复率最高的是处理9(1 g/L活性炭+2 g/L聚乙烯醇+166 mg/L钙离子),恢复率为89.07%,其次是处理4(0.5 g/L活性炭+166mg/L钙离子),81.05%、处理8(1 g/L活性炭+1 g/L聚乙烯醇),73.87%。方差分析表明,活性炭、聚乙烯醇、钙离子浓度对草莓玻璃化苗恢复的影响都极为显著,影响大小依次是钙离子>活性炭>聚乙烯醇。恢复苗的各项生理生化指标与玻璃化苗差异显著,与正常苗差异不显著。结合各处理对玻璃化苗的恢复率、生理生化指标及增殖系数,综合考虑认为使草莓玻璃化试管苗恢复的最佳处理为处理9。
The objective of this study is to explore the prevention and recovery measures of strawberry vitrification shoots.Using method of orthogonal design,different combinations of activated carbon,PVA,calcium ion（calcium chloride）were added to subculture medium of the vitrification shoots of‘Toyonoka＇strawberry to study the effects of strawberry recovery rate.Meanwhile,the physiological and biochemical indexes among recovery plantlets,vitrification shoots and normal plantlets were compared.There were significant differences in recovery rate of vitrification shoots among 9 treatments.The optimum one was No.9（1g/L activated carbon ＋ 2 g/L PVA ＋ 166 mg/L calcium ion）and the recovery rate was 89.07%,followed by the 4thtreatment（0.5 g/L activated carbon ＋ 166 mg/L calcium ion）,81.05%,and the 8thtreatment（1 g/L activated carbon ＋ 1 g/L polyvinyl alcohol）,73.87%,respectively.Analysis of variance showed that calcium ion concentration was the most significant impact factor on strawberry vitrification recovery,the second was calcium ion,and polyvinyl alcohol affected least.Significant different physiological and biochemical indexes between the recovery plantlets and vitrification shoots were observed but not between recovery plantlets and normal plantlets.Combined with the recovery rate of vitrification shoots,physiological and biochemical indexes,and the reproduction coefficient,the best recommended recipe for vitrification shoots recovery was the 9th.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第9期89-93,共5页
Biotechnology Bulletin
关键词
草莓
正交设计
玻璃化试管苗
恢复
生理生化
Strawberry Orthogonal design Vitrification shoots Recovery Physiology and biochemistry
