摘要
目的对2型糖尿病并发泌尿系感染的病原菌分布及耐药性进行观察分析,为临床合理使用抗菌药物提供参考依据。方法以常规方法分离病原菌,沉渣划线法接种血平板、麦康凯平板,置孵育箱35℃培养24h后鉴定菌种,并对其做药敏试验。结果 291例标本共分离出296株细菌,同时检出两种菌株5例,其中革兰阴性菌149株占50.34%,以大肠埃希菌为主,占40.88%;革兰阳性菌120株占40.54%,以表皮葡萄球菌和金黄色葡萄球菌为主,分别占14.52%和8.11%;真菌27株占9.12%;296株病原菌中27株真菌未做药敏试验,其余269株病原菌中大肠埃希菌对青霉素类耐药性较高,青霉素G耐药性为95.04%,表皮葡萄球菌、金黄色葡萄球菌和肺炎克雷伯菌对氨苄西林耐药性最高均为100.0%,粪肠球菌对红霉素耐药性最高为100.0%。结论 2型糖尿病患者并发泌尿系感染发病率较高,病原菌多为革兰阴性菌和革兰阴性菌,真菌感染有所增加,耐药菌株增加,应根据病原菌种类及药敏结果,合理筛选抗菌药物。
OBJECTIVE To observe and analyze the distribution and antibiotic resistance of the pathogens causingurinary tract infections in patients with type 2 diabetes so as to provide the evidence for clinical rational use of anti-microbial agents. METHODS The pathogens were isolated by routine method,the blood agar and MacConkey platewere inoculated through sediment crossed method, which were then placed in 35 °C incubator for 24 hours,andthe species of pathogens were identified, the drug susceptibility testing was performed. RESULTS Totally 296strains of bacteria were isolated from the 291 specimens,there were 5 cases with two species detected. There were149 （50. 34%） strains of gram-negative bacilli, among which the Escherichia coli was dominant, accounting for40. 88% ; there were 120 （40. 54%） strains of gram-positive bacteria, among which the Staphylococcus epidermidis and Staphylococcus aureus were dominant, accounting for 14. 52% and 8. 11%, respectively; there were 27（9. 12%） strains of fungi. Of 296 strains of pathogen, there were 27 strains of fungi which were not taken thedrug susceptibility testing; of the rest of 269 strains of pathogens,the E. coli was highly resistant to the penicil-lins, the drug resistance rate to penicillin G was 95. 04% ; the drug resistance rates of the S. epidermidis, S. aureus, and Klebsiella pneumoniae to ampicillin were the highest （100. 0%） ; the drug resistance rate of the Entero-coccus faecalis to erythromycin was the highest （100. 0%）. CONCLUSION The incidence of urinary tract infectionsis high in the patients with type 2 diabetes,the gram-negative bacteria and the gram-negative bacteria are dominantamong the pathogens, the cases with fungal infections are increased,and the drug resistant strains are increased. Itis necessary to reasonably use antibiotics based on the species of pathogens and the result of drug susceptibility testing.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2013年第17期4308-4309,4335,共3页
Chinese Journal of Nosocomiology
基金
沈阳市卫生局科研项目(2011-03)
关键词
2型糖尿病
泌尿系感染
病原菌分布
Type 2 diabetes； Urinary tract infection； Distribution of pathogen
