摘要
将犬瘟热病毒（CDV）接种Vero细胞和DF1细胞进行传代并测定其病毒滴度,选取细胞病变出现早、病毒滴度高的Vero细胞毒作为接种病毒抗原免疫蛋鸡。通过每10d进行蛋鸡免疫1次,四免后每30d加强免疫1次的方法进行免疫。免疫前、后每10d采血分离血清和每5d收集鸡蛋提取卵黄抗体,用琼脂扩散法和间接ELISA法测定其抗体水平。结果显示,血清抗体比卵黄抗体的滴度高,两者间呈正相关性,卵黄抗体出现较血清抗体晚3~5d,卵黄抗体在三免后3~5d就已达到较高水平,此时即可开始收集鸡蛋制备卵黄抗体。
To study the immune response,dynamics and its correlation analysis of the serum and egg yolk antibody in laying hens against CDV antigen,and to provide the basis for the preparation of egg yolk antibody.Vero cell and DF1 cell cultures were inoculated with CDV,to detect the virus titer.Select the Vero cytotoxic strain with earlier cytopathic and high virus titers as inoculated virus antigen to immunize laying hens.For four times once every 10 days,then to strengthen the immune every 30 days.Blood serum was separated every 10 days and egg yolk antibody was collected every 5 days before and after immunization,the antibody levels was determined by agar diffusion and the indirect ELISA.The results showed that serum antibody titer was higher than yolk antibody and both were positively correlated;the appearance of yolk antibody was later 3 to 5 days than that of serum antibody.Egg yolk antibody reached the highest level at 3 to 5 days after three immunity,and at this time to collect eggs and to prepare yolk antibody.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第9期1319-1322,共4页
Chinese Journal of Veterinary Science
基金
山东省优秀中青年科学家科研奖励基金资助项目(BS2011SW026)
关键词
犬瘟热病毒
血清抗体
卵黄抗体
消长规律
canine distemper virus
serum antibody
egg yolk antibody
dynamics
