摘要
目的:探讨2010年版《中国药典》山楂叶中总黄酮含量的测定方法。方法:以芦丁为对照品,用紫外-可见分光光度法测定山楂叶中总黄酮的含量,并结合高效液相色谱法考察山楂叶中总黄酮含量测定方法的准确性。结果:B环无邻二酚羟基结构的黄酮类化合物,采用芦丁显色紫外-可见分光光度法测定总黄酮含量误差较大,也不是总黄酮含量测定的专属方法。结论:芦丁显色紫外-可见分光光度法测定山楂叶中总黄酮含量的方法值得商榷,建议将芦丁显色法和高效液相色谱法结合起来测定,这样会更科学、准确。
OBJECTIVE：To discuss the method for the content determination of flavonoids in Crataegi Folium in Chinese Pharmacopoeia（2010 edition）.METHODS：Using rutin as control,the content of flavonoids in Crataegi Folium was determined by ultraviolet-visible spectrophotometry,and the accuracy of the method was investigated by combined with HPLC.RESULTS：There were biggish error to determine the flavonoids content of structure without adjacent diphenol hydroxyl in B benzene ring by the ultraviolet-visible spectrophotometry,and it was also not the exclusive method.CONCLUSIONS：It is questionable to determine the content of total flavonoids in Crataegi Folium by the rutin colorimetric ultraviolet-visible spectrophotometry.So we suggest to use the method of rutin colorimetric method combined with HPLC.It is more scientific and accurate.
出处
《中国药房》
CAS
CSCD
2013年第35期3309-3311,共3页
China Pharmacy
基金
承德市科学技术研究与发展计划项目(No.20101312)
关键词
山楂叶
芦丁
总黄酮
紫外-可见分光光度法
高效液相色谱法
中国药典
商榷
Crataegi Folium
Rutin
Total flavonoids
Ultraviolet-visible spectrophotometry
HPLC
Chinese Pharmacopoe ia
Discussion
