摘要
目的:探讨原儿茶酸(protocatechuic acid,PCA)及其衍生物体外清除DPPH自由基活性及对鸡胚绒毛尿囊膜(chick chorioallantoic membrane,CAM)血管新生的影响。方法:利用苄基保护及苄酯水解等相关反应,得到PCA系列衍生物PCA-1,PCA-2,PCA-3,结构经1H,13C-NMR及MS验证,并采用DPPH自由基体外抗氧模型、CAM对PCA及其衍生物的活性进行筛选。结果:PCA及含有邻二酚羟基的PCA-1的体外清除DPPH自由基活性较强;PCA,PCA-3对新生血管有一定的抑制作用,而PCA-2则能促进血管的新生(P<0.001)。结论:PCA分子结构中的邻二酚羟基为体外清除DPPH自由基的活性部位;不含邻二酚羟基,同时有羧基时能增强其促血管新生活性,但需要进一步的实验验证。
To explore the effects of protocatechuic acid （PCA） and its derivants on angiogenesis of the chick embryo chorioal- lantoic membrane （CAM） and scavenging DPPH radical in vitro. The protection of benzyl and alkaline hydrolysis of benzyl ester were employed. The structures of PCA-1, PCA-2 and PCA-3, the derivates of PCA , were elucidated by ^1H, ^13C-NMR and MS data. The bioaetivity of PCA and its derivants was evaluated on the models of DPPH radical and chick embryo chorioallantoic membrane （CAM） , respectively. PCA and PCA-1 showed the best activity of scavenging DPPH radical among all the compounds. In contrast to PCA-2, PCA and PCA-3 displayed inhibition to angiogenesis （P 〈 0. 001 ）. Pyrocatechol hydroxyl is the active site of PCA on scavenging DPPH radical in vitro. PCA with carboxyl and without pyrocatechol hydroxyl seems to show promotion to angiogenesis, but it needs more evidences.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第2期208-211,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81173519)
北京中医药大学创新团队项目(2011-CXTD-15)
关键词
原儿茶酸
衍生物
DPPH自由基
鸡胚绒毛尿囊膜
生物活性
protocatechuic acid
derivants
DPPH radical
chick embryo chorioallantoic membrane （CAM）
bioactivity
