摘要
目的探讨免疫抑制治疗对受体源性的再上皮化过程的影响,以及免疫抑制治疗的最佳方案。方法将60只大鼠随机分为6组,每组10只,建立大鼠气管异位移植模型。对照组1:大鼠气管内注入生理盐水;对照组2:大鼠气管内注入受体上皮细胞悬液;实验组1:大鼠气管内注入受体上皮细胞悬液,移植后每日肌肉注射他克莫司(FK506)0.5mg/kg,共3d;实验组2:大鼠气管内灌注受体上皮细胞悬液,移植后每日肌肉注射FK506 0.5mg/kg,共10d;实验组3:气管内灌注受体上皮细胞悬液,移植后每日肌肉注射FK506 1.0mg/kg,共3d;实验组4:气管内灌注受体上皮细胞悬液,移植后每日肌肉注射FK506 1.0mg/kg,共10d。移植后第28天,处死大鼠,取气管制备标本,分别行苏木精-伊红(HE)染色、淋巴细胞浸润计数,上皮细胞CK14、CK18和CFTR分化鉴定,以及透射电镜观察,观察各组大鼠气管异位移植再上皮化的情况。结果对照组1未见任何上皮细胞再生,管腔出现严重狭窄、闭塞;对照组2也未实现再上皮化,但管腔狭窄、闭塞程度稍好于对照组1;实验组1和实验组2气管内壁仅局部有上皮细胞再生,且细胞尚未分化成熟;实验组3和实验组4受体源性再上皮化取得成功,气管内覆盖了完整连续的成熟的纤毛柱状上皮细胞,并具有腺体分泌功能、屏障防御功能。结论气管内注入受体上皮细胞后再实施异位移植,在一定的条件下可实现受体源性的完全再上皮化。
Objective To study the influence of immunosuppressive therapy on re-epithelization from the recipient, and the preferred plan of the therapy. Methods Sixty rats were evenly randomized to six groups, and a heterotopic subcutaneous tracheal transplantation model in rat was created. Control group 1： intratracheal injection of normal saline; Control group 2： intra- tracheal injection of recipient epithelial cells suspension （RECS） ： Experimental group 1 ： intratracheal iniection of RPCS, and, after transplantation, I.M. injection of Tacrolimus （0.5 mg · kg ^-1 · d^-1）, daily for 3 days; Experimental group 2： intratracheal injec- tion o5 RPCS, and with LM. Injection 05 Tacr01imus （0.5 mg· kg ^-1 · d^-1） for 10 days after transplantation; intramuscular injec- tion of Tacrolimus （0.5 mg· kg ^-1 · d^-1） for ten days; Experimental group 3： intratracheal injection of RPCS, and I.M. injection of Tacrolimus （1.0 mg· kg ^-1 · d^-1） for 3 days after transplantation; Experimental group 4： intratracheal injection of RPCS, and I.M. injection of Tacrolimus （1.0 mg · kg ^-1 · d^-1） for 10 days after transplantation. The rats were killed on day 28 after trans- plantation, the implanted tracheas were harvested. The histological and ultrastruetural changes, infiltrated lymphocytes and differ- entiation of CK14, CK18, and CFTR were evaluated. Results In the control group 1, no any epithelial cell regeneration was ob- served, and severely narrowed and occluded lumen appeared; in the control group 2, no epithelization was observed as well, but the condition of the lumen was a slightly better than that in the former group; in experimental groups 1 and 2, only local epithelial re- generation, hut immature, could be seen; in experimental groups 3 and 4, the epithelization was succeeded. The inner surface of trachea was covered by completely well-developed epithelial ceils with gland secretion function and barrier function. Conclusion A heterotopic tracheal grafting after intratracheal injection of RPCS, under a certain condition, a complete re-epithelization from the recipient can be realized.
出处
《青岛大学医学院学报》
CAS
2013年第6期487-491,共5页
Acta Academiae Medicinae Qingdao Universitatis
基金
上海市科委资助项目(114119a9400)
关键词
气管
移植
上皮细胞
免疫抑制剂
trachea
transplantation
epithelial cells
immunosuppressive agents
