摘要
重链抗体(heavy chain antibody,HcAb)是存在于骆驼和鲨鱼类动物体内,轻链天然缺失,只有重链组成的新型抗体分子。重链抗体和普通的抗体相比虽然缺失了轻链,但是依然保留结合抗原的能力。通过基因工程的改造获得仅有可变区,但保留结合能力的新型工程抗体——单域抗体(single-domain antibody),也被称为纳米抗体(nanobody),具有相对分子质量小、稳定性高、易表达等优点;同时,克服了人源抗体改造后亲和力降低、易聚集等缺点,成为抗体药物研究领域的新型抗体分子。
Heavy-chain antibodies constitute a major fraction of the functional antibodies in the serum of camelids. They are devoid of light chains and maintain the specificity and affinity to antigens. Through genetic engineering techniques, single-domain antibody （also called nanobody） exhibits certain advantages such as low molecular weight, better stability as well as easy expression etc. In addition, through overcoming the limitations of low affinity and easy-to-aggregation, nanobody becomes one of the prestigious subtypes in the research and development of therapeutic antibodies.
出处
《生命科学》
CSCD
2013年第9期853-857,共5页
Chinese Bulletin of Life Sciences
基金
卫生部“重大新药创制”科技重大专项课题(2011ZX09506-001)
关键词
重链抗体
纳米抗体
抗体药物
heavy chain antibody
nanobody
therapeutic antibody
