摘要
目的通过对大连市市区部分正常中老年人肠道菌群情况的检查结果统计分析,为该市正常中老年人肠道菌群正常指标提供参考依据。方法取不同年龄段的老年人粪便,分别采用显微镜检查和微生物培养方法,进行肠道菌群分析。选择涂片均匀的大便标本,用油镜计数500个细菌,计算革兰阳性菌和革兰阴性菌的比例。结果正常中年人肠道菌群具体比率情况报告如下:革兰阴性杆菌:55.5±5.7,革兰阳性球菌19.9±5.8,革兰阳性杆菌17.2±5.0;老年人的肠道菌群具体比率情况报告如下:革兰阴性杆菌69.5±6.4,革兰阳性球菌13.1±4.9,革兰阳性杆菌14.9±5.9,真菌孢子0.1±0.1。经过粪便标本菌群分析,老年组的双歧杆菌数量明显下降。结论年龄等因素对肠道菌群有影响,临床诊断与检验应考虑不同年龄层次的人群。
Objective To establish the standard of normal intestinal flora proportion by analyzing intestinal flora of normal middle- aged and aged people in Dalian. Methods Uniform feces smears were chosen to count 500 bacteria under microscope, and then the per- centages of gram-negative and gram-positive bacteria were calculated. Results The percentages of gram-negative bacilli, gram-positive cocci and gram-positive bacilli in the intestinal flora of normal middle-aged people were 55.5±5.7, 19.9 ± 5.8 and 17.2 ± 5.0 respec- tively, and the percentages of gram-negative bacilli, gram-positive cocci, gram-positive bacilli and fungal spore of intestinal flora in the aged people were 69.5 ± 6.4, 13.1 ± 4.9, 14.9 ± 5.9 and 0.1 ± 0. 1 respectively. Conclusion The normal intestinal flora proportion is affected by age and other physiologic factors, which should be taken into consideration during clinical diagnosis and examination.
出处
《中国微生态学杂志》
CAS
CSCD
2013年第9期1097-1098,共2页
Chinese Journal of Microecology
关键词
中老年人
肠道菌群
菌群失调
Middle-aged and aged people
Intestinal flora
