摘要
目的 观察肝硬化患者肠道菌丛潜生体 (CGC)和群集形成情况 ;了解细菌CGC形成是否与肝功能分级有关 ;探讨某些药物对肠道细菌CGC和群集形成的影响。方法 受试者包括 86例肝硬化患者 (实验组 )和 2 0例健康人 (对照组 ) ,用细菌形态学检测方法观察粪便中有无细菌CGC和群集 ;用细菌显微培养技术、细菌遗传稳定性试验和最低抑菌浓度测定观察CGC的生物学特点 ;观察药物对细菌CGC和群集的影响。结果 肝硬化患者的粪便中细菌CGC和群集率 (5 0 0 % )显著高于正常人 (5 0 % ) (P <0 0 1) ,肝硬化患者的CGC具有传代生长、活动能力强和抗生素耐受力强的特点。肝功能越差 ,细菌CGC形成率越高 (P <0 0 5 ) ,细菌CGC和群集阳性的患者分别用氟哌酸、米雅BM片和乳果糖治疗后 ,CGC和群集率分别为 90 9%和 6 3 6 % ,5 0 0 %和 33 3% ,71 1%和 0 ,与安慰剂比较 ,氟哌酸组对CGC形成率的影响无明显差异 (P >0 0 5 ) ,米雅BM片和乳果糖则差异有显著性(P <0 0 5 )。结论 肝硬化患者的肠道细菌CGC形成率和群集率可显著增高。肝硬化患者的CGC有很强的繁殖能力、运动能力和抗生素耐受力 ,从而具有极强的侵袭力。乳果糖及生态制剂较抗生素在调节细菌CGC形成和群集方面更理想。
Objectives To investigate the formation and regulation of cryptic growth cells(CGC) of the intestinal flora in patients with liver cirrhosis and effects of some drugs on the formation of CGC Methods The experimental group consisted of 86 patients with liver cirrhosis and 20 healthy subjects as the control Bacterial morphological examination was employed to identify whether there were CGC and aggregation in the stool specimens; microculture technique, bacterial genetic stability test and MIC were used to observe the biological characteristics of CGC; and the therapeutic effects of drugs on CGC and bacterial aggregation were evaluated randomly Results It was found that the incidence of positive CGC and aggregation in the stool was far higher in the cirrhotic patients (50 0%) than in the control (5 0%)( P <0 01) The CGC in the cirrhotic patients was characterized by its generative ability in the next generation with high activity and powerful tolerance towards antibiotics When compared with placebo,the effects of norfloxacin were not significant ( P >0 05),but MayiBM tablets and lactulose showed very significant effects ( P <0 05) Conclusions The formation rate of CGC and aggregation of the intestinal flora was significantly increased in patients with liver cirrhosis; the CGC possessed powerful aggressive ability; lactulose and other biological preparations are more ideal to inhibit the formation of CGC and aggregation of the intestinal flora in cirrhotic patients
出处
《中华内科杂志》
CAS
CSCD
北大核心
2000年第4期251-254,共4页
Chinese Journal of Internal Medicine
基金
国家自然科学基金!资助项目 (39870 346)
